The curious world of apoptotic cell clearance
© BioMed Central Ltd 2004
Published: 29 September 2004
Analysis of knockout mice has brought into question the previously proposed role of the phosphatidylserine receptor (Ptdsr) in the clearance of apoptotic cell corpses, and has suggested important functions in regulating differentiation and inflammation.
In order to be recognized for removal, dying cells present signals at the cell surface that trigger engulfment either by professional phagocytes (macrophages) or by amateurs (neighboring cells). The best known of these signals is the phospholipid phosphatidylserine (PS) . A large number of proteins have been reported to bind to exposed PS molecules on dying cells; some bind to PS directly and some via bridging molecules. Working out why there are so many PS-binding proteins and how they all work is a major preoccupation of apoptosis researchers.
A few years ago, Valerie Fadok, Peter Henson and colleagues, at the National Jewish Medical and Research Center in Denver, Colorado, generated monoclonal antibodies that prevent phagocytosis by human macrophages . The antibodies also stimulated the production of transforming growth factor-β (TGF-β) and blocked the production of the inflammatory cytokine tumor necrosis factor-α (TNF-α), suggesting a link between PS recognition and downregulation of the inflammatory response after the uptake of apoptotic cells. Henson's group used phage display to clone the antigen recognized by one of these antibodies, mAb 217 . They reported that the mAb 217 recognized a predicted transmembrane PS receptor that was similar to homologs in Caenorhabditis elegans and Drosophila melanogaster, suggesting conservation of function. Henson and colleagues proposed this gene as a good candidate for a PS-specific receptor that is critical for mediating the uptake of apoptotic cells, adding a cautionary note that "we cannot rule out at this time that it facilitates PS recognition by some other function which does not involve direct binding to PS" .
The PS receptor knockouts
Lengeling's group was not the only one keen to figure out what the PS receptor does: at least two other groups were also generating and characterizing a mouse knockout for the PS receptor (Ptdsr) [5, 6]. But comparison of the reports from the different groups is puzzling. Li et al.  concluded that the PS receptor is essential for the removal of apoptotic cells and saw defective phagocytosis of apoptotic cells by macrophages derived from their Ptdsr-knockout mice. They also found an accumulation of dead cells in the lung and brain, which they suggested could explain the observed neonatal lethality. A few months later, Kunisaki et al.  reported that erythropoiesis and T-cell lymphopoiesis were blocked in their Ptdsr-knockout mouse strain, and that this mutation also resulted in impaired clearance of apoptotic cells in the liver and thymus.
Lengeling's group was bemused; they could find no evidence for impaired clearance using several different techniques, both in vitro and in vivo, looking at different organs and developmental stages. But their mice had so many other phenotypes that they had their hands full . There was severe perinatal lethality and a large number of defects in different tissues, all of which were related to delayed differentiation. Embryos were growth-retarded, with malformations of the head, palate, and the developing eye. The Ptdsr-deficient embryos also had a delay in tissue differentiation in the lung, kidney, and intestine. Brain hyperplasia and a block in erythropoietic differentiation were also observed, as in the reports from Li et al.  and Kunisaki et al. , respectively. One of the most striking defects was the absence of eyes in some embryos associated with the induction of ectopic eye structures in nasal cavities. Finally, they observed reduction in the levels of macrophage cytokines that had not been reported by the other groups.
Reconciling the results
The existence of several knockouts of the same gene with very different phenotypes is puzzling, intriguing, and divides researchers in the field about how to interpret the results. Simon Brown of the University of Edinburgh, UK, urges readers to focus on the commonality of the three studies. "All three found the homozygous-null mouse to be perinatal lethal with clear evidence of a defect in cell differentiation and marked effects on tissue and organ development following the mid-gestation period," he notes. He admits that there are some major differences that draw one's attention but suspects these can be explained by differences in experimental approaches. Most researchers seem to agree that Lengeling's analysis is particularly careful, but the discrepancies between the different studies remain perplexing.
Michael Hengartner at the University of Zurich, Switzerland, is unequivocal. "Lengeling's results clearly demonstrate that Ptdsr is most certainly not a PS receptor, and probably has nothing to do with apoptotic cell recognition at all." He suspects that the source of the problem is a case of false identification during the expression-cloning of the antigen recognized by mAb 217. This is supported by the supplementary data provided by Böse et al. , in which mAb 217 is shown to recognize macrophages derived from their knockout mouse . "One wonders why the other two groups did not perform this control using their mice," notes Hengartner. Shigekazu Nagata from the Osaka University Medical School, Japan, also feels that the cloned Ptdsr gene had not been sufficiently characterized previously. "Lengeling's group now shows that Ptdsr carries an epitope that can be weakly recognized by the mAb 217. But the antibody efficiently stains even the Ptdsr-deficient cells, indicating that the antibody recognizes a molecule other than Ptdsr."
"The PS receptor story is an interesting case of how an excusable error, that can easily happen in any scientific pursuit, results in a series of published data that are guided by prejudice," says Angelika Böttger from the Ludwig-Maximilians-Universität in Munich, Germany. She is sure that the mAb 217 antibody really does inhibit the phagocytosis of apoptotic cells. "The only problem is that the Ptdsr gene does not encode the antigen for this antibody." She says that the experiment in which the Ptdsr-deficient cells are stained with the mAb 217 "should finally convince everybody that the dogma is wrong."
Siamon Gordon, a macrophage expert at the University of Oxford, UK, feels that "the main point of this article is that people were not looking hard enough before and were jumping to conclusions." But Henson himself welcomes the different results. "The more studies we have on this molecule the more interesting it gets, and it clearly has multiple functions." Henson confides that his group has generated a fourth knockout strain and preliminary results suggest that the phenotypes differ from the other three. He admits that it appears confusing, but is confident that the data will eventually fit together." Gordon suggests that different cells may contribute to clearance in different scenarios. "Macrophages are faster and more efficient professional phagocytes than non-leukocytes, so they may be the main players during inflammation or infection." He notes that earlier studies had indicated that macrophages are not essential for apoptotic clearance during development, adding that C. elegans has no macrophages but can still clear corpses.
More surprises in store
Many experts hope that clues will come from analysis of the Ptdsr protein in other species. Xue's group has analyzed the role of the Ptdsr gene in worms and found evidence that it is involved in removing apoptotic cells . But Kristin White's group at MGH-Harvard in Charlestown, USA, has studied the PS receptor in flies and came up with results that fit more with those in the Lengeling article. "We see no obvious defect in engulfment of apoptotic cells in Drosophila embryos that lack PS receptor. These animals are viable, with some subtle developmental defects," says White.
Future studies will obviously focus on functions of the Ptdsr beyond apoptotic cell clearance. Nagata hopes that the Lengeling study will trigger investigation of the 'real' function of Ptdsr during mammalian development. "These have little in common; thus Ptdsr probably has a specific cellular role that is required at multiple occasions throughout development," says Hengartner. White agrees: "Since we also see effects on development in the fly PS receptor mutant, this suggests that PS receptor has a biological function that is more general than the recognition of apoptotic cells. The use of different biological systems and approaches to dissect the role of this protein should help us to understand this more general role."
There are likely to be more surprises in store. "The Ptdsr protein carries a domain called the Jumonji C (JmjC) domain," notes Nagata, speculating that, like other proteins with this domain, Ptdsr may play a role in chromatin remodeling and the stability of heterochromatin. Edinburgh's Brown makes similar predictions and indeed, there is evidence that the Ptdsr protein may be located in the nucleus [8, 9]. Böttger, whose lab studies the PS receptor from Hydra, suggests "it could modify nuclear proteins, transcription factors or maybe proteins maintaining nuclear architecture and thus have these profound effects on differentiation during early mouse development."
Alice's adventures came to an end when she awoke from her dream. But our apoptotic adventures are likely to continue for some time as we learn more about the surprises that govern when and how cells die and who is responsible for clearing up the remains.
- Carroll L: Alice's Adventures in Wonderland. 1865, London: Macmillan
- Böse J, Gruber AD, Helming L, Schiebe S, Wegener I, Hafner M, Beales M, Köntgen F, Lengeling A: The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal. J Biol. 2004, 3: 15-10.1186/jbiol10.PubMedPubMed CentralView Article
- Fadok VA, Voelker DR, Campbell PA, Cohen JJ, Bratton DL, Henson PM: Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol. 1992, 148: 2207-2216.PubMed
- Fadok VA, Bratton DL, Rose DM, Pearson A, Ezekewitz RA, Henson PM: A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature. 2000, 405: 85-90. 10.1038/35011084.PubMedView Article
- Li MO, Sarkisian MR, Mehal WZ, Rakic P, Flavell RA: Phosphatidylserine receptor is required for clearance of apoptotic cells. Science. 2003, 302: 1560-1563. 10.1126/science.1087621.PubMedView Article
- Kunisaki Y, Masuko S, Noda Z, Inayoshi A, Sanui T, Harada M, Sasazuki T, Fukui Y: Defective fetal liver erythropoiesis and T lymphopoiesis in mice lacking the phosphatidylserine receptor. Blood. 2004, 103: 3362-3364. 10.1182/blood-2003-09-3245.PubMedView Article
- Wang X, Wu YC, Fadok VA, Lee MC, Gengyo-Ando K, Cheng LC, Ledwich D, Hsu PK, Chen JY, Chou BK: Cell corpse engulfment mediated by C. elegans: phosphatidylserine receptor through CED-5 and CED-12. Science. 2003, 302: 1563-1566. 10.1126/science.1087641.PubMedView Article
- Cikala M, Alexandrova O, David CN, Pröschel MBS, Cramer P, Böttger A: The phosphatidylserine receptor from Hydra is a nuclear protein with potential Fe(II)-dependent oxygenase activity. BMC Cell Biol. 2004, 5: 26-10.1186/1471-2121-5-26.PubMedPubMed CentralView Article
- Cui P, Qin B, Liu N, Pan G, Pei D: Nuclear localization of the phosphatidylserine receptor protein via multiple nuclear localization signals. Exp Cell Res. 2004, 293: 154-163. 10.1016/j.yexcr.2003.09.023.PubMedView Article