Trees in the Web of Life
© BioMed Central 2009
Published: 13 July 2009
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© BioMed Central 2009
Published: 13 July 2009
Reconstructing the 'Tree of Life' is complicated by extensive horizontal gene transfer between diverse groups of organisms. While numerous conceptual and technical obstacles remain, a report in this issue of Journal of Biology from Koonin and colleagues on the largest-scale prokaryotic genomic reconstruction yet attempted shows that such a tree is discernible, although its branches cannot be traced.
The Tree of Life (ToL) is a widely used metaphor to describe the history of life on Earth. While Darwin argued that the 'Coral of Life' may be a more apt description (since only the surface remains alive, supported by the dead generations beneath it), relationships between organisms based on shared characters are best organized using the schematic representation of a tree. Use of molecular markers, in particular small-subunit ribosomal RNA, have allowed this metaphor to be extended to microorganisms; however, this has also presented unique challenges for notions of phylogeny and evolution. One of the most significant challenges is the impact of horizontal gene transfer, which causes genes that coexist in a genome to have different molecular phylogenies . Despite these challenges, the increasing ease with which genomes can be sequenced has reinvigorated attempts to use genomic information to reconstruct the ToL.
All microbial individuals arise as the result of a fission of a parent individual. Therefore, a vertical line of descent exists, and could theoretically be reconstructed as a purely bifurcating tree (that is, an organismal or cytoplasmic tree). However, while evolution presupposes and requires descent via reproduction, the two are not analogous. Evolution is, by definition, the change in the genetic material within a population of organisms across generations; therefore, any process by which genetic material within a population changes that is unrelated to the reproduction of individuals will show a history that is unrelated to the organismal vertical line of descent. This includes horizontal gene transfer. In many cases, the sum effect of these other genetic processes may completely obfuscate vertical descent, leaving only some measure of 'relatedness' based on overall genetic similarity.
If stringent criteria are applied to remove or down-weigh transferred genes from supertree or supermatrix analyses, the resulting trees at best represent the history of only a minor fraction of the genome, largely consisting of ribosomal proteins, effectively a 'tree of one percent' . Even if this remaining 'genome core' retains a strong signal of vertical descent, this does not capture the true evolutionary history of genomes; that is, a web where different strands depict the history of different genes. A ribosomal tree of life has other shortcomings, in that within taxonomic orders many recombination and lineage sorting events may occur, and ribosomal genes are so highly conserved that such events at the tips of the tree may not be detectable. However, it can still provide a useful backbone for a reticulated genomic or organismal phylogeny [10, 11], especially with respect to sets of genes that clearly have undergone horizontal transfer between more distantly related groups. While ribosomal protein and RNA encoding genes have been transferred in the past (see discussion in ), these genes are resistant to transfer , with most transfers occurring between close relatives. These properties make a phylogenetic reconstruction using ribosomal RNA and proteins an ideal scaffold upon which to map horizontal gene transfers, clearly depicting their distinct contribution to genomic (and organismal) evolution. Several attempts have been made to capture this web-like genome history (see, for example, [10, 11] using ribosomal rRNA as a backbone (Figure 1). Conceptually, this method is distinct from any 'tree of one percent'  or genome averaging approach in that rather than being discarded, genes undergoing horizontal transfer are included in the final reconstruction without obscuring the vertical signal, even if that vertical signal is preserved only in a minority of genes.
In this issue, Puigbo, Wolf and Koonin  present an approach for salvaging the ToL that is a variant on other supertree methods, in which nearly 7,000 phylogenetic trees of prokaryotic genes (a 'Forest of Life') are compared in order to determine a central tendency in their topologies. The trees are built from clusters of orthologous groups of proteins (COGs), and the central tendency is deduced from a set of nearly universal trees (NUTs), defined by Puigbo et al. as those trees generated from a set of COGs that are represented in >90% of the analyzed prokaryote taxa. What distinguishes their approach from earlier supertree analyses – apart from the very large number of genes included in the comparison – is that it does not depend on a concatenation of highly conserved proteins or rRNAs, or on a supertree generated by 'pruning' down to those genes giving a consistent topology, to determine a central tendency. Instead, Puigbo et al. calculate an 'inconsistency score' that is a measure of how representative a particular topology of each tree is to the rest of the trees in the Forest of Life.
In reconstructing the central tendency in such a broad distribution of gene phylogenies, the work by Puigbo et al. also shows the difficulty in resolving deep branches, which often simply collapse into radiations without any topological structure. In confronting this problem, they show that the relationship between phylogenetic depth and resolution supports a tree-like structure for these deep branches. This result is significant in that it suggests that there is no need to postulate exotic 'big bang' radiations early in evolution; rather, deep phylogenies can still be represented as bifurcating evolutionary events, albeit with extremely short branches that can prove difficult (or sometimes impossible) to resolve.
Integrating the vertical descent of organisms and their genomes with the myriad phylogenetic patterns produced by horizontal gene transfer is essential for a truly comprehensive understanding of evolution. A new method that acknowledges and promotes this integration, even if falling short of fully encompassing the intricate details of a complex genome-based biological reality, represents progress towards this goal, and it now appears that a vertical signal can be discerned, if not clearly resolved.
Work in the authors' lab is supported through the NSF Assembling the Tree of Life (DEB 0830024) and NASA exobiology (NAG5-12367 and NNX07AK15G) programs.