Figure 7

Representative z-stack of TUNEL⁺/doublecortin⁺ cells. Photographs were taken at 2 μm intervals. Identical analyses were conducted for every cell that was scored as TUNEL⁺ and expressing a cell type-specific antigen, as shown in Figure 4. Each row shows, from left to right, TUNEL staining, doublecortin staining, S-100b staining, and the merged image. (a-d) Images taken at -4 μm; (e-h) -2 μm; (i-l) 0 μm; (m-p) 2 μm. The congruence between the doublecortin⁺ staining and the TUNEL⁺ nuclei (which shows up as yellow in the merged image) was presumably due to the changes in antigen distribution associated with nuclear fragmentation, as this was always cell-type specific in that there was overlap only in those cases in which the rest of the cell was also stained with the same antibody. For example TUNEL⁺/doublecortin⁺ cells were always doublecortin⁺ in the cytoplasm, and other antibodies used in the same sections did not label the TUNEL-labeled nuclei of doublecortin⁺ cells.