Figure 4

Systemic 5-FU exposure causes prolonged suppression of proliferation in the adult CNS. Animals were treated as described in Figure 3. The number of BrdU⁺ cells was analyzed in control animals and 5-FU-treated animals and presented as percentage normalized values of controls at each time point. For ease of comparison, data presented in the figures show the control value (mean set at 100%) of day 1 and normalized values of 5-FU treatment groups at all time points. Each group consisted of n = 5; a two-way ANOVA test was performed on the original un-normalized data set to test the statistical significance of treatment effect and time effect. Bonferroni post-tests were performed to compare the 5-FU-treated group and the control group at each time point. The statistical significance of the Bonferroni post-tests is labeled in the graphs where applicable: ***p < 0.001; **p < 0.01; or *p < 0.05. Two-way ANOVA test results indicate that: (a) in the SVZ, both the treatment effect and time effect are extremely significant (p < 0.001), and the interaction of treatment and time is very significant (p < 0.01); (b) in the CC, both the treatment effect and time effect are extremely significant (p < 0.001), and the interaction of treatment and time is very significant (p < 0.01); and (c) in the DG, the treatment effect is very significant (p < 0.01), the time effect is extremely significant (p < 0.001), and the effect of the interaction between treatment and time is not significant.