Figure 5

Growth-deficient phenotypes of DInr, Dp110 and dPKB mutants are suppressed by loss of dFOXO. (a) Control fly. (b) Selective removal of DInr from the head leads to a pinhead phenotype, which is partially suppressed by the loss of dFOXO (c). The same suppression is observed in Dp110-, and dPKB-pinheads (d-g). The TSC1^-/- bighead phenotype (h) is enhanced by mutations in dFOXO (i), but the dPTEN^-/- bighead (j) is slightly suppressed (k). (l) Living without PKB. In contrast to the larval lethality of dPKB null mutants, dPKB-dFOXO double mutants develop into small pharate adults, most of which fail to eclose. Bar sizes are 200 μm (low magnification) and 20 μm (high magnification). Genotypes are: (a) y w ey-flp/y w; FRT82/FRT82 cl3R3 w⁺; (b) y w ey-flp/y w; FRT82 DInr³⁰⁴/FRT82 cl3R3 w⁺; (c) y w ey-flp/y w; FRT82 DInr³⁰⁴EP-dFOXO²⁵/FRT82 cl3R3 w⁺; (d) y w ey-flp/y w; FRT82 Dp110^5W3/FRT82 cl3R3 w⁺; (e) y w ey-flp/y w; FRT82 Dp110^5W3EP-dFOXO²⁵/FRT82 cl3R3 w⁺; (f) y w ey-flp/y w; FRT82 dPKB¹/FRT82 cl3R3 w⁺; (g) y w ey-flp/y w; FRT82 dPKB¹EP-dFOXO²⁵/FRT82 cl3R3 w⁺; (h) y w ey-flp/y w; FRT82 dTSC1^Q87X/FRT82 cl3R3 w⁺; (i) y w ey-flp/y w; FRT82 dTSC1^Q87XEP-dFOXO²⁵/FRT82 cl3R3 w⁺; (j) y w ey-flp/y w; FRT40 dPTEN^117-4/FRT40 cl2L3 w⁺; (k) y w ey-flp/y w; FRT40 dPTEN^117-4/FRT40 cl2L3 w⁺; FRT82 EP-dFOXO²⁵/FRT82 cl3R3 w⁺; (l) y w;; EP-dFOXO²¹/EPdFOXO²⁵ (left), y w;; dPKB¹EP-dFOXO²¹/dPKB¹EP-dFOXO²⁵ (middle), dPKB¹/dPKB¹ (right).