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Table 1 Overview of identified molecular targets

From: Systematic identification of regulatory proteins critical for T-cell activation

Known to function in TCR pathway
Gene Domain homology Direction Accession number* Relative to ORF* Frequency* Phenotype transfer
TCRβ Receptor Sense Numerous Partial 46 On hold
ZAP70 Tyrosine kinase Sense L05148.1 -147, +787 nt 12 Yes
ZAP70 (long) Tyrosine kinase Sense L05148.1 -21, +809 nt 17 TBD
Syk Tyrosine kinase Sense L28824.1 -27, +1012 nt 2 Yes
Lck Tyrosine kinase Sense U23852.1 -59, +799 nt 4 Yes
PLCγ1 Tyrosine kinase Sense NM_002660.1 +1409, +2282 nt 3 Yes
SHP-1/PTP1C Protein-tyrosine phosphatase Sense X62055.1 +472, >+2021 nt 1 Yes
Csk Tyrosine kinase Sense NM_004383.1 -55, +1285 nt 1 TBD
PAG Transmembrane adaptor Sense NM_018440.2 -237, +644 nt 1 Yes
Nucleolin RNA-binding Sense NM_005381.1 -136, +479 nt 1 No
Enzymes and receptors
TCPTP/PTPN2 Protein-tyrosine phosphatase Sense NM_002828.1 -58, +1108 nt 20 Yes
PAK2 p21-activated kinase 2 Sense NM_002577.1 -50, +339 nt 18 Yes
PAK2 (long) p21-activated kinase 2 Sense NM_002577.1 -42, +670 nt 1 Yes
A-Raf-1 Serine/threonine kinase Sense X04790.1 -4, +456 nt 5 Yes
EDG1 G-protein-coupled receptor Sense NM_001400.2 <-244, +942 nt 4 Yes
EDG1 (long) G-protein-coupled receptor Sense NM_001400.2 <-244, +1037 nt 1 TBD
TRAC-1 RING finger ubiquitin ligase Sense NM_017831.1 -254, +510 nt 1 Yes
IL-10Rα Receptor Sense NM_001558.1 +689, +1350 nt 1 Yes
Integrin α2 Receptor Sense NM_002203.2 +3348, +3914 nt 1 Yes
Enolase 1α Phosphopyruvate hydratase Sense NM_001428.1 +703, +1374 nt 2 No
DUSP1 Dual-specificity phosphatase Sense NM_004417.2 +817, +1112 nt 1 No
KIAA0251 Pyridoxal-dependent decarboxylase Sense D87438.1 nt 2098-2370 1 No
Adaptors and transcription factors
Grb7 Adaptor Sense NM_005310.1 +1268, +1912 nt 3 Yes
GG2-1 TNF-induced protein Sense AF070671.1 -97, +1795 nt 2 Yes
SH2-B Adaptor Sense AF227968.1 +1352, +1960 nt 1 Yes
RERE Transcriptional factor Sense AB036737.1 +914, +1202 nt 3 No
SudD Serine/threonine rich Sense NM_003831.1 -93, +413 nt 1 No
Ku 70 DNA-PKc subunit Sense S38729.1 +1026, +2069 nt 1 No
Novel (130 amino acids; no homology) Unknown Sense AC005321.1 nt 33543-33938 1 No
Novel signaling molecule GYF domain Sense NM_022574.1 +1, +121 nt 1 No
SCAMP2 Secretory carrier membrane protein Sense AF005038.2 -5, +833 nt 1 No
KIAA1228 C2 domain (Ca2+- or IP-binding) Sense AB033054.2 nt 1439-2163 1 No
EST from clone 2108068 LPP20 lipoprotein precursor Sense Al357532.1 Novel isoform 1 No
RNH Ribonuclease/angiogenin inhibitor Sense NM_002939.1 -22, +713 nt 1 No
Cytoskeleton
Moesin Moesin Sense NM_002444.1 -93, +1534 nt 1 Yes
Vimentin Intermediate filament Sense NM_003380.1 -98, +374 nt 1 Yes
Others       
Alu repeat      5 On hold
CpG island? (clone 550H1)    AL035420.1   1 On hold
IgG2 heavy chain Ig superfamily Sense   Partial 1 On hold
Ig light chain Ig superfamily Sense   Partial 1 On hold
18S rRNA   Sense M10098.1 Partial 2 On hold
28S rRNA   Sense   Partial 1 On hold
  1. *For each identified clone, the GenBank database [51] accession number is given, followed by the first and last nucleotide (nt) positions relative to the initiation codon (ATG being the +1, +2, +3 nts, respectively); Frequency indicates the number of original cell clones expressing the specific hit. Relative to the EST itself because the start codon is not identified. Relative to the genomic clone itself. Ig, Immunoglobulin; TBD, to be determined.
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