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Table 1 Screens for lamellar and cytokinesis phenotypes in cultured Drosophila cells

From: Functional genomics of cell morphology using RNA interference: pick your style, broad or deep

Phenotype: Cytokinesis Lamellar Actin/shape
Cell type: S2 S2 S2R, Kc167 S2 cells S2R, Kc167
Study: Somma et al. [9] Rogers et al. [10]; Goshima and Vale [7] Kiger et al. [8] Rogers et al. [10] Kiger et al. [8]
Protein (gene)*      
Myosin-II (zipper) NT + - - -
Myosin RLC (spaghetti squash) + NT + NT -
Profilin (chickadee) - + - + +
CAP (act up/capulet) NT + - + +
Cofilin (twinstar) + + + + +
Actin interacting protein 1; Aip1 NT + NT + NT
Formin (diaphanous) NT + + - +
Anillin (scraps) + + + - +
Kinesin (pavarotti) + + + - -
Rho1 GTPase + + + + -
Rho GEF (pebble) + NT + NT -
RacGAP; RacGAP50C + + - - -
Syntaxin1A + NT NT NT NT
Dynamin (shibire) NT NT + NT -
Citron kinase NT + + - -
Aurora kinase NT NT + NT +
Arp2/3 complex NT - - + +
Scar NT - - + +
Cortactin NT - - - +
Nck (dock) NT - - + -
Cdc42 GTPase NT - - + +
Rac1/2 GTPase NT - - - + (Rac2 only)
Capping protein NT - - + +
Abl kinase NT - - + +
Abelson-interacting protein (abi) NT - - + -
Enabled kinase NT - - - +
Rho kinase NT NT - NT +
PAK NT - (PAK1) - - + (PAK3)
Cofilin phosphatase (slingshot) NT - - + +
Rho GEF (vav) NT - - - +
PIP3 phosphatase (pten) NT NT - NT +
Other myosins NT - - - + (uncharacterized ORF, CG15780)
Polo kinase NT NT + NT +
LIM kinase NT - - - -
Ciboulot NT - - - -
Coronin NT - NT - NT
Filamin NT - - (jitterbug) - - (jitterbug)
Gelsolin NT - - - -
Tropomodulin (sanpodo) NT - - - -
Tropomyosin NT - NT - NT
Septin (peanut) - NT NT NT NT
WASP NT - - - -
  1. + indicates that a cytokinesis or lamellar phenotype was observed; - no phenotype was observed; NT, not tested. *Protein names (sometimes more than one alternative) are given in roman type and gene names in italics. In unpublished experiments, accumulation of actin and disruption of the cell monolayer was found with profilin RNAi and a cytokinesis phenotype was seen with zipper RNAi (A. Kiger, personal communication). Mitotic phenotypes were observed.