Table 1 Screens for lamellar and cytokinesis phenotypes in cultured Drosophila cells
From: Functional genomics of cell morphology using RNA interference: pick your style, broad or deep
Phenotype: | Cytokinesis | Lamellar | Actin/shape | ||
|---|---|---|---|---|---|
Cell type: | S2 | S2 | S2R, Kc167 | S2 cells | S2R, Kc167 |
Study: | Somma et al. [9] | Rogers et al. [10]; Goshima and Vale [7] | Kiger et al. [8] | Rogers et al. [10] | Kiger et al. [8] |
Protein (gene)* | Â | Â | Â | Â | Â |
Myosin-II (zipper) | NT | + | -†| - | -†|
Myosin RLC (spaghetti squash) | + | NT | + | NT | - |
Profilin (chickadee) | - | + | -†| + | +†|
CAP (act up/capulet) | NT | + | - | + | + |
Cofilin (twinstar) | + | + | + | + | + |
Actin interacting protein 1; Aip1 | NT | + | NT | + | NT |
Formin (diaphanous) | NT | + | + | - | + |
Anillin (scraps) | + | + | + | - | + |
Kinesin (pavarotti) | + | + | + | - | - |
Rho1 GTPase | + | + | + | + | - |
Rho GEF (pebble) | + | NT | + | NT | - |
RacGAP; RacGAP50C | + | + | - | - | - |
Syntaxin1A | + | NT | NT | NT | NT |
Dynamin (shibire) | NT | NT | + | NT | - |
Citron kinase | NT | + | + | - | - |
Aurora kinase | NT | NT | + | NT | + |
Arp2/3 complex | NT | - | - | + | + |
Scar | NT | - | - | + | + |
Cortactin | NT | - | - | - | + |
Nck (dock) | NT | - | - | + | - |
Cdc42 GTPase | NT | - | - | + | + |
Rac1/2 GTPase | NT | - | - | - | + (Rac2 only) |
Capping protein | NT | - | - | + | + |
Abl kinase | NT | - | - | + | + |
Abelson-interacting protein (abi) | NT | - | - | + | - |
Enabled kinase | NT | - | - | - | + |
Rho kinase | NT | NT | - | NT | + |
PAK | NT | - (PAK1) | - | - | + (PAK3) |
Cofilin phosphatase (slingshot) | NT | - | - | + | + |
Rho GEF (vav) | NT | - | - | - | + |
PIP3 phosphatase (pten) | NT | NT | - | NT | + |
Other myosins | NT | - | - | - | + (uncharacterized ORF, CG15780) |
Polo kinase | NT | NT | +‡ | NT | +‡ |
LIM kinase | NT | - | - | - | - |
Ciboulot | NT | - | - | - | - |
Coronin | NT | - | NT | - | NT |
Filamin | NT | - | - (jitterbug) | - | - (jitterbug) |
Gelsolin | NT | - | - | - | - |
Tropomodulin (sanpodo) | NT | - | - | - | - |
Tropomyosin | NT | - | NT | - | NT |
Septin (peanut) | - | NT | NT | NT | NT |
WASP | NT | - | - | - | - |