Figure 3

RNAi screens identified a wide range of gene functions based on diverse morphological phenotypes. Cells were stained for F-actin (red), α-tubulin (green) and DNA (blue), imaged using automated microscopy and scored visually. (a) Control Kc₁₆₇ cells. (b-e) Kc₁₆₇ cells with RNAi phenotypes. (f) Control S2R⁺ cells. (g-j) S2R⁺ cells with RNAi phenotypes. (b) F-actin accumulation; CG13503 RNAi (encoding a predicted WH2-containing actin-binding protein). (c, h) Flatter, polarized cells with actin-rich lamellipodia (arrows); CG5169 RNAi (a predicted kinase). (d) Opposing protrusions rich in F-actin (arrow) or microtubules (arrowhead), Hsp83 RNAi (chaperone). (e) Flat cells; puckered RNAi (JNK phosphatase). (g) Widely-distributed F-actin puncta; capping protein beta RNAi (component of CapZ). (i) Radial protrusions (arrows) and reduced cortical actin (asterisk); CG31536 RNAi (predicted Rho-GEF with FERM domain). (j) Rounder cells, decreased in size; CG4629 RNAi (predicted kinase). Scale bar, 30 μm.