Figure 6From: A functional genomic analysis of cell morphology using RNA interferenceRNAi profiles identify known and novel genes with related morphogenetic functions. Table headings are as defined as in Figure 2. (a, b) Profile I: binucleate cells that identified genes required for cytokinesis, as detected either in (a) both cell types or (b) a single cell type. (c, d) Profile II: F-actin phenotypes observed in both cell types identified genes with potentially conserved roles in F-actin dynamics. (c) Increased or polarized (uneven) accumulation of F-actin identified genes with potential roles in F-actin capping, severing or depolymerization. (d) Reduced F-actin and altered cell shape identified genes with potential roles in F-actin polymerization. (e, f) Profile III: a common RNAi phenotype observed in Kc167 cells was a change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. (e) Cases with phenotypes also observed in S2R+ cells identified genes involved in F-actin and microtubule regulation. (f) Cases with phenotypes observed only in Kc167 cells identified components of receptor signaling pathways. (g-i) Profile IV: RNAi phenotypes resulting in round, detached S2R+ cells. (g) Phenotypes detected in both S2R+ and Kc167 cells identified genes with probable indirect effects on cell adhesion and spreading, including roles in the cell cycle and cell viability; (h) RNAi phenotypes specific for S2R+ cells identified genes that may distinguish the flat S2R+ cell morphology, including genes encoding cell-matrix adhesion components. (i) Genes identified by a related RNAi phenotype, resulting in retracted (unspread but flat) S2R+ cells.Back to article page