Journal of Biology

Table 1 RNAi screen results classified by predicted gene function

From: A functional genomic analysis of cell morphology using RNA interference

		Genes identified*
Gene class^†	N	Total		Total S2R⁺		Total Kc₁₆₇		Both^‡
Kinase	229	54	18.8%	3	18.8%	2	12.5%	2	12.5%
Miscellaneous	139	16	11.5%	15	10.8%	11	7.9%	10	7.2%
Cytoskeletal	116	17	14.7%	17	14.7%	11	9.5%	11	9.5%
Motor	77	7	9.1%	6	7.8%	3	3.9%	2	2.6%
Phosphatase	72	12	16.7%	10	13.9%	7	9.7%	5	6.9%
GTPase	54	15	27.8%	15	27.8%	6	11.1%	6	11.1%
Transport	48	2	4.2%	1	2.1%	2	4.2%	1	2.1%
Proteolysis	42	7	16.7%	7	16.7%	6	14.3%	6	14.3%
Lipid-associated	38	3	7.9%	3	7.9%	0	0%	0	0%
GEF	32	8	25.0%	7	21.9%	3	9.4%	2	6.3%
PDZ	32	3	9.4%	3	9.4%	1	3.1%	1	3.1%
GAP	31	6	19.4%	5	16.1%	2	6.5%	1	3.2%
SH2/SH3	25	3	12.0%	2	8.0%	1	4.0%	0	0%
Adhesion	23	3	13.0%	3	13.0%	0	0%	0	0%
Cyclase	20	1	5.0%	0	0%	1	9.5%	0	0%
G protein	16	3	18.8%	3	18.8%	2	12.5%	2	12.5%
Total genes	994	160	16.1%	146	14.7%	79	7.9%	65	6.5%

In total, we screened 1,061 wells, 1,042 dsRNAs, 994 genes and found 160 genes with phenotypes. *The number and percentage of genes identified with any RNAi phenotype in duplicate screens. ^†The total number of genes (N) represented in the dsRNA set as defined by amino-acid sequence and Gene Ontology [33] or FlyBase [12] annotation. Each gene was counted in only one category. ^‡Genes identified by phenotypes in both Kc₁₆₇ and S2R⁺ cells.

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