Figure 1

Mapping the binding domains in Mo-MuLV Gag and human endophilin 2 using the yeast two-hybrid system. Yeast strain CTY 10-5d was transformed with different combinations of DNAs encoding the fusion proteins. Fragments of Mo-MuLV Gag were fused to the DNA-binding domain of pSH2LexA, whereas endophilin 2 was fused to the activation domain of pGADNOT. (a) Domains in Mo-MuLV Gag assayed for binding to endophilin 2. (b) Domains in endophilin assayed for binding to Mo-MuLV Gag. The scoring of β-galactosidase activity of yeast colonies is as follows: -, no blue color after 24 h in reaction; +/-, blue after 8 h; +, blue after 2 h; ++, blue between 30 min and 2 h; +++, blue in approximately 30 min; ++++, blue between 15 and 30 min. All constructs tested negative for self-activation. Quantitation of β-galactosidase levels was as described previously [71].