Figure 10

Effects on the release of virion-associated capsid of overexpression of endophilin 2 and its fragments. Plasmid (5 μg) encoding HA-tagged endophilin 2 fragments was transfected into 293T cells with either (a) 1 μg of Mo-MuLV proviral DNA (pNCA) or (c) 1 μg of HIV-1 codon-optimized gag-pol DNA construct CMVgagpolBNkan. In (b), 293T cells were cotransfected with 1 μg of Mo-MuLV proviral DNA (pNCS) and endophilin 2 at ratios of 1:0, 1:1, 1:2.5 and 1:5. The total DNA for each transfection was normalized at an amount of 6 μg by addition of empty vector plasmid. Equal amounts of cell lysate and virion particles were analyzed by western blotting with anti-HA (top panels in a, b and c), anti-Mo-MuLV capsid (middle and bottom panels in a and b) or anti-p24 antibodies (c, middle and bottom panels).