Figure 3
From: Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production
Incorporation of HA-endophilin 2 into Mo-MuLV virions. The 293T cells were transiently transfected with 2 μg of plasmid encoding HA-tagged endophilin 2, either alone or together with 10 μg of proviral DNA, as indicated. The proteins in cell lysates and purified virions were analyzed by western blotting with (a,c) anti-capsid and (b,d) anti-HA antibodies. Cells were transfected with plasmids expressing (e) HA-p11 (annexin II light chain) or (f) HA-endophilin 2 along with Mo-MuLV DNA, and serial dilutions of cell lysates and virion proteins were analyzed by western blot with anti-HA antisera.