Figure 7
From: Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production
Endogenous endophilin 2 and other endocytic proteins are incorporated into Mo-MuLV virions. The 293T cells were either mock-transfected or transfected with a proviral DNA, pNCS. Equal amounts of cell lysates and virion particles were analyzed by western blotting with various antisera: (a) anti-endophilin 2; (b) anti-α-adaptin (a subunit of AP-2); (c) anti-clathrin; (d) anti-dynamin 2. Two of these incorporated proteins were shown to be protected from protease digestion within the virions after treatment with increasing levels of subtilisin: (e) α-adaptin and (f) clathrin. Under these conditions, the viral envelope protein is digested while the internal CA protein is protected.