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Table 1 Interactions between endophilin 2 and retroviral Gag proteins in the yeast two-hybrid system

From: Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production

 

pGADNOT-AD fusion

pSH2-LexA fusion

Endophilin 2

Gag

Mo-MuLV Pr65 Gag

+++

++++

RSV Pr65 Gag

++/+

++++

MPMV Pr76 Gag

-

++

SIV Pr57 Gag

-

+++

HIV-1 Pr55 Gag

-

++++

Human endophilin 2

++++

 
  1. The various Gag proteins were fused to the DNA-binding domain, and human endophilin 2 was fused to the activation domain. All constructs tested negative for self-activation. Symbols: -, no blue color after 24 h in reaction; +, yeast turn blue after 2 h; ++, blue develops between 30 min and 2 h; +++, blue in approximately 30 min; ++++, blue between 15 and 30 min.
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Journal of Biology

ISSN: 1475-4924