Comparisons of CENP-C proteins in animals, yeast and plants. The CENPC motif and conserved regions found at the termini of CENP-C proteins are indicated. For pairwise comparisons of protein-coding sequences, regions of positive and negative selection between the species compared are shown. (a) Alignment of animal and fungal CENP-Cs. Mammalian CENP-Cs align throughout their lengths, as do the two Saccharomyces Mif2p proteins, but others align only at conserved regions. Portions of the human CENP-C protein implicated in centromere-targeting (purple bars) and DNA-binding (black bars) are shown at the top. The scale bar at the top marks the length of human CENP-C in amino acids. (b) Alignment of plant CENP-Cs. Within angiosperm families, proteins align throughout their lengths. Between families, weak conservation is found at the amino terminus and strong conservation at the carboxyl terminus. (c) Logos representation of an alignment of the CENPC motif from human; mouse; cow; chicken; Caenorhabditis elegans; budding yeast; Schizosaccharomyces pombe; Physcomitrella patens; maize CenpcA; rice; A. thaliana; black cottonwood, soybean, and tomato.