Figure 1

Specific targeting of memory and regulatory CD4⁺ T cells by Tnfrsf4-driven Cre expression. (a) Activated YFP expression in a subset of splenic (SP) and lymph node cells (LN) isolated from Tnfrsf4^Cre/+ R26^Yfp/+ mice. Numbers within dot plots denote the percentage of YFP⁺ cells. (b) Percentage (mean ± SEM, n = 6-9) of CD4⁺, CD8⁺ or CD19⁺ cells or cells negative for all three markers (other) in gated YFP⁺ cells from the spleen and lymph nodes of Tnfrsf4^Cre/+R26^Yfp/+ mice. (c, d) Percentage (mean ± SEM, n = 6-9) of YFP⁺ cells in (c) total, naïve (CD44^loCD25^-), memory (CD44^hiCD25^-) and regulatory (reg; CD25⁺) CD4⁺ T cells and in (d) total, naïve (CD44^loCD25^-) and memory (CD44^hiCD25^-) CD8⁺ T cells, both from the spleen and lymph nodes of Tnfrsf4^Cre/+R26^Yfp/+ mice. (e) Percentage (mean ± SEM, n = 4-8) of YFP⁺ cells in naïve, memory and regulatory CD4⁺ T cells from Tnfrsf4^Cre/+ R26^Yfp/+ (YFP/+) and Tnfrsf4^Cre/+ R26^Yfp/Dta(YFP/DTA) mice. (f) Flow cytometric example of YFP and CD134 induction 1 day (d1) after in vitro stimulation of sorted naïve YFP^- CD4⁺ T cells (d0) from Tnfrsf4^Cre/+ R26^Yfp/+ mice. (g) Percentage of YFP⁺ and CD134⁺ cells (mean ± SEM, n = 4-6) in CD4⁺ T cells stimulated as in (f). (h) Percentage of annexin V⁺ cells following in vitro activation of sorted naïve CD4⁺ T cells from Tnfrsf4^Cre/+R26^Dta/+ (DTA) or control Tnfrsf4^Cre/+R26^+/+ (WT) mice.