Figure 7

Influence of microbial exposure on immune homeostasis. (a) Serum LBP levels (mean ± SEM, n = 6-9) in Ikbkg^fl/YVil-Cre (NEMO) and littermate control Ikbkg^fl/Y(WT) mice. The number above the bars is the P-value. (b) Total numbers (mean, n = 8-9) of B cells, T cells and macrophages (Mphi) in Ikbkg^fl/YVil-Cre (NEMO) and littermate control Ikbkg^fl/Y(WT) mice. P = 0.0007 for macrophages. (c) Flow cytometric example of CD11b⁺F4/80⁺ macrophage expansion in the spleen of NEMO mice, compared with WT mice. (d) CD4:CD8 ratio (± SEM) in the same mice. (e) Total numbers of naïve, memory and regulatory (reg) CD4⁺ T cells and naïve and memory CD8⁺ T cells from NEMO and control WT mice. (f) Serum LBP levels (mean ± SEM, n = 9-13) in Tnfrsf4^Cre/+ R26^Dta/+ (DTA) and control Tnfrsf4^Cre/+ R26^+/+ (WT) mice. (g) Total numbers (mean, n = 4-6) of B cells, T cells and macrophages (Mphi), (h) CD4:CD8 ratio and (i) total numbers of naïve, memory and regulatory (reg) CD4⁺ T cells and naïve and memory CD8⁺ T cells in Tnfrsf4^Cre/+ R26^Dta/+MyD88^-/- (DTA) and littermate control Tnfrsf4^Cre/+ R26^+/+ MyD88^-/- (-) mice. P = 0.035 for total cells; P = 0.015 for B cells; P = 0.0003 for regulatory CD4⁺ T cells; P = 0.018 for total CD8⁺ T cells; and P = 0.0003 for memory CD8⁺ T cells. (j) CD62L and CD43 expression in gated memory CD44^hiCD8⁺ T cells from the same mice. Numbers within bars denote the absolute number, ×10^-7 in (b, g), and ×10^-6 in (e, i), of each cell type.