Endogenous Dsh shuttles between the cytoplasm and nucleus. Immunofluorescent staining of HEK293 cells with anti-Dvl2 antibodies reveals different subcellular localization of Dvl2 (a) without or (b) with LMB treatment. (c) DAPI staining shows the location of nuclei in the same field as (b); the arrowheads indicate corresponding nuclei in (b) and (c). (d) Distribution of endogenous Dvl2 recognized by anti-Dvl2 antibodies in the nuclear and the cytoplasmic fractions of Rat-1 fibroblasts. In the absence of NEM, Dvl2 is localized mainly in the cytoplasm (C), while after NEM treatment Dvl2 is exclusively localized in the nuclei (N). W, whole cell lysate. Antibodies to lamin and GAPDH show the separation of the nuclear and cytoplasmic fractions.