Figure 4

Mapping nuclear localization signals in Dsh. (a) The Dsh constructs used to study nuclear transport and their localization to the nucleus after NEM or LMB treatment; the DIX, PDZ and DEP domains are shown as in Figure 1a; B is the basic region and nd denotes not done. (b-i) Subcellular localization of Dsh-GFP constructs in the absence or presence of NEM or LMB. Embryos were injected with 0.5 ng of each mRNA, and GFP analysis was carried out as in Figure 1b-d. (b-d) Ds1, (e-g) DsNLSm, (h) Dsh, (i) DsSNLS. (b,e,i) no NEM treatment; (c,f) after NEM treatment; (d,g,h) after LMB treatment. (j) Comparison of conserved amino-acid sequences that are required for Dsh nuclear localization; X denotes the Xenopus protein, m the mouse and h the human. Amino-acid residues mutated in DsNLSm are indicated by asterisks.