Nuclear translocation of Dvl2 upon Wnt3a treatment. (a) MCF7 cells were treated either with Wnt3a-conditioned or control medium for 6 h, fixed and immunostained with anti-Dvl2 antibodies. In control cells, cytoplasmic and perinuclear staining is visible. Wnt3a-conditioned, but not control, medium enhanced nuclear translocation of Dvl2. DAPI staining indicates the position of cell nuclei. Corresponding cells are shown by arrowheads. (b) Nuclear/cytoplasmic (N/C) ratios of fluorescence were calculated for each panel in (a) as the mean +/- standard deviation.