Co-overexpression of Madm and bunA causes overgrowth. (a-d) Scanning electron micrographs of adult eyes as a readout for the consequences of overexpression of bunA and Madm under the control of the GMR-Gal4 driver line late during eye development. Whereas expression of (b) bunA or (c) Madm singly does not cause a size alteration compared to the control (a), overexpression of both leads to increased eye size (d). (e) The size increase on bunA and Madm coexpression is due to larger ommatidia (Student's t-test, n = 9, ***p < 0.001). (f-i) The growth-promoting effect of bunA and Madm co-overexpression is also observed in the wing. Single expression of either (g, g') bunA or (h, h') Madm during wing development (by means of C10-Gal4) does not change wing size or curvature visibly. However, their combined expression causes a slight overgrowth of the tissue between the wing veins, resulting in a wavy wing surface and wing bending (i'), manifested as folds between wing veins in (i) (arrows). Genotypes are: (a) y, w; GMR-Gal4/UAS-eGFP; UAS-lacZ/+; (b) y, w; GMR-Gal4/UAS-eGFP; UAS-bunA/+; (c) y, w; GMR-Gal4/UAS-Madm; UAS-lacZ/+; (d) y, w; GMR-Gal4/UAS-Madm; UAS-bunA/+; (f) y, w; UAS-eGFP/+; C10-Gal4/UAS-lacZ; (g) y, w; UAS-eGFP/+; C10-Gal4/UAS-bunA; (h) y, w; UAS-Madm/+; C10-Gal4/UAS-lacZ; (i) y, w; UAS-Madm/+; C10-Gal4/UAS-bunA.