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Figure 7 | Journal of Biology

Figure 7

From: Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs

Figure 7

Binding of deacylated tRNA to the E site. (a) Binding of [33P]-tRNAfMet to the postT complex with fMet-Phe-Ile-tRNAIlein the P site and a Thr codon (ACG) in the E site. Insert: Scatchard plot to obtain the dissociation constant. (b) Chase of [33P]-tRNAfMet from the E site of postT complexes containing Met (AUG), Phe (UUU) or Thr (ACG) codons with unlabeled tRNAPhe. Dissociation constants for [33P] tRNAfMet were obtained as in (a) and the dissociation constants for tRNAPhe were calculated from the 50% chase (I50) concentrations of tRNAPhe.

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