Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs

Journal of Biology

Table 1 Dissociation constants for the binding of tRNA^fMet and tRNA^Phe to different ribosomal complexes

Set	State	Peptide	Codon in the E site	tRNA	Dissociation constant K_D (nM)	Additional factors; temperature
1	PreT	fMI	Thr (ACG)	fMet	144 ± 7	37°C
					179 ± 14	EF-G + GTP; 37°C
					770 ± 80	EF-G + GDP; 37°C
					40 ± 2	EF-G + GDPNP; 37°C
2	PostT	fMI	Met (AUG)	fMet	153 ± 9	37°C
				Phe	250 ± 30
		fM	Phe (UUU)	fMet	295 ± 26
				Phe	84 ± 11
		fMFTI	Thr (ACG)	fMet	162 ± 5
				Phe	134 ± 20
3	PostT	fMI	Met (AUG)	fMet	155 ± 10	+ RelE (A-site cut); 37°C
4	PostT	fMI	Met (AUG)	fMet	24.8 ± 1.2	0°C

Different conditions were used for measuring the dissociation constants for the different combinations of tRNA and ribosomal complexes as inFigure 7, and are shown in the last column.