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Table 1 Dissociation constants for the binding of tRNAfMet and tRNAPhe to different ribosomal complexes

From: Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs

Set State Peptide Codon in the E site tRNA Dissociation constant KD (nM) Additional factors; temperature
1 PreT fMI Thr (ACG) fMet 144 ± 7 37°C
      179 ± 14 EF-G + GTP; 37°C
      770 ± 80 EF-G + GDP; 37°C
      40 ± 2 EF-G + GDPNP; 37°C
2 PostT fMI Met (AUG) fMet 153 ± 9 37°C
     Phe 250 ± 30  
   fM Phe (UUU) fMet 295 ± 26  
     Phe 84 ± 11  
   fMFTI Thr (ACG) fMet 162 ± 5  
     Phe 134 ± 20  
3 PostT fMI Met (AUG) fMet 155 ± 10 + RelE (A-site cut); 37°C
4 PostT fMI Met (AUG) fMet 24.8 ± 1.2 0°C
  1. Different conditions were used for measuring the dissociation constants for the different combinations of tRNA and ribosomal complexes as inFigure 7, and are shown in the last column.