Table 1 Selected genes up- or down-regulated in transgenic lungs
From: Hyperactive Wnt signaling changes the developmental potential of embryonic lung endoderm
Affymetrix probe ID | Allele | Gene name | GenBank accession numbers | Â | Fold change (transgenic/ wild-type) | p value |
|---|---|---|---|---|---|---|
Genes down-regulated in transgenic lungs | ||||||
Specialized cell markers | ||||||
1418818_at | Aqp5 | aquaporin 5 | Mm.45580 | NM_009701 | 96.1 | 0.009 |
1449015_at | Retnla | resistin like alpha | Mm.33772 | NM_020509 | 37.5 | 0.004 |
1436996_x_at | Lyzs | lysozyme | Mm.45436 | AV066625 | 36.5 | 6.82E-04 |
1451537_at | Chi3l1 | chitinase 3-like 1 | Mm.4376 | BC005611 | 30.5 | 0.002 |
1429626_at | Sftpa | surfactant associated protein A | Mm.46062 | AV024301 | 30.1 | 0.004 |
1420504_at | Slc6a14 | solute carrier family 6 (neurotransmitter transporter)14 | Mm.25770 | AF320226 | 21.4 | 0.023 |
1419020_at | Gif | gastric intrinsic factor | Mm.456 | NM_008118 | 19.7 | 0.003 |
1416456_a_at | Chia | chitinase, acidic | Mm.46418 | BC011134 | 16.0 | 0.012 |
1423547_at | Lyzs | lysozyme | Mm.45436 | AW208566 | 14.8 | 0.004 |
1419764_at | Chi3l3 | chitinase 3-like 3 | Mm.4571 | NM_009892 | 13.6 | 0.013 |
1420378_at | Sftpd | surfactant associated protein D | Mm.1321 | BC003705 | 12.0 | 2.10E-04 |
1448553_at | Myh7 | myosin, heavy polypeptide 7, cardiac muscle, beta | Mm.155714 | NM_080728 | 11.7 | 0.011 |
1455431_at | Slc5a1 | solute carrier family 5 (sodium/glucose cotransporter)1 | Mm.25237 | AV371434 | 10.4 | 0.025 |
1437028_at | Sftpb | surfactant associated protein B | Mm.46033 | AV025094 | 9.8 | 5.41E-04 |
1438696_at | Edn3 | endothelin 3 | Mm.9478 | BB368452 | 7.0 | 0.016 |
1452543_a_at | Scgb1a1 | secretoglobin, family 1A, member 1 (uteroglobin) | Mm.2258 | X67702 | 5.2 | 0.014 |
1425291_at | Foxj1 | forkhead box J1 | Mm.4985 | L13204 | 2.3 | 0.006 |
Genes up-regulated in transgenic lungs | ||||||
Cell proliferation markers | ||||||
1451417_at | Brca1 | breast cancer 1 | Mm.1889 | U31625 | 9.5 | 0.004 |
1416969_at | Gtse1 | G two S phase expressed protein 1 | Mm.20858 | NM_013882 | 5.9 | 7.58E-04 |
1416122_at | Ccnd2 | cyclin D2 | Mm.3141 | NM_009829 | 3.5 | 1.27E-04 |
1422460_at | Mad2l1 | MAD2 (mitotic arrest deficient, homolog)-like 1 (yeast) | Mm.43444 | NM_019499 | 3.2 | 0.014 |
1425166_at | Rbl1 | retinoblastoma-like 1 (p107) | Mm.2994 | U27178 | 3.1 | 0.007 |
1417420_at | Ccnd1 | cyclin D1 | Mm.22288 | BB538325 | 2.2 | 0.002 |
Transcription factors and Notch/Delta signaling | ||||||
1421299_a_at | Lef1 | lymphoid enhancer binding factor 1 | Mm.200634 | NM_010703 | 58.7 | 1.29E-04 |
1426552_a_at | Bcl11a | B-cell CLL/lymphoma 11A (zinc finger protein) | Mm.24020 | BB772866 | 19.5 | 0.001 |
1417679_at | Gfi1 | growth factor independent 1 | Mm.2078 | NM_010278 | 18.0 | 0.038 |
1449236_at | Dll3 | delta-like 3 (Drosophila) | Mm.12896 | AB013440 | 16.7 | 0.001 |
1424903_at | Smcy | selected mouse cDNA on the Y | Mm.1064 | AF127244 | 10.8 | 0.006 |
1451835_at | Sox21 | SRY-box containing gene 21 | Mm.70950 | AY069926 | 8.9 | 0.001 |
1449822_at | Atoh1 | atonal homolog 1 (Drosophila) | Mm.57229 | BC010820 | 7.4 | 0.001 |
1422914_at | Sp5 | transacting transcription factor 5 | Mm.155690 | NM_022435 | 7.1 | 0.043 |
1448595_a_at | Rex3 | reduced expression 3 | Mm.14768 | NM_009052 | 6.5 | 2.55E-04 |
1450164_at | Ascl1 | achaete-scute complex homolog-like 1 (Drosophila) | Mm.10663 | NM_008553 | 6.1 | 0.007 |
1460336_at | Ppargc1 | peroxisome proliferative activated receptor, gamma, coactivator 1 | Mm.10707 | BB745167 | 5.7 | 0.039 |
1418055_at | Neurod4 | neurogenic differentiation 4 | Mm.10695 | NM_007501 | 5.2 | 0.017 |
1424950_at | Sox9 | SRY-box containing gene 9 | Mm.46607 | BI077717 | 5.1 | 0.019 |
1450339_a_at | Bcl11b | B-cell lymphoma/leukaemia 11B | Mm.116831 | NM_021399 | 5.0 | 0.042 |
1460214_at | Pcp4 | Purkinje cell protein 4 | Mm.5023 | NM_008791 | 4.5 | 0.012 |
1415811_at | Np95 | nuclear protein 95 | Mm.42196 | BB702754 | 4.0 | 0.037 |
1419437_at | Sim2 | single-minded 2 | Mm.4775 | NM_011377 | 3.9 | 0.03 |
1415810_at | Np95 | nuclear protein 95 | Mm.42196 | NM_010931 | 3.9 | 0.017 |
1451255_at | Lisch7 | liver-specific bHLH-Zip transcription factor | Mm.4067 | BC004672 | 3.7 | 8.53E-04 |
1421951_at | Lhx1 | LIM homeobox protein 1 | Mm.4965 | NM_008498 | 3.3 | 0.014 |
1417302_at | Rcor | RE1-silencing transcription factor (REST) co-repressor | Mm.22980 | NM_054048 | 3.3 | 0.047 |
1422088_at | Lmyc1 | Mus musculus, clone IMAGE:1528432, mRNA | Mm.198846 | BI687857 | 3.0 | 0.001 |
1424942_a_at | Myc | myelocytomatosis oncogene | Mm.2444 | BC006728 | 2.6 | 7.85E-04 |
1417155_at | Nmyc1 | neuroblastoma myc-related oncogene 1 | Mm.16469 | BC005453 | 2.3 | 0.01 |
Specialized cell markers | ||||||
Paneth cells | ||||||
1450709_at | Defcr5 | defensin related cryptdin 5 | Mm.140173 | NM_007851 | 844.0 | 0.003 |
1416905_at | Guca2 | guanylate cyclase activator 2 (guanylin 2, intestinal, heatstable) | Mm.2614 | NM_008190 | 322.2 | 0.001 |
1450631_x_at | Defcr6 | defensin related cryptdin 6 | Mm.246485 | NM_007852 | 187.6 | 0.002 |
1427119_at | Spink4 | serine protease inhibitor, Kazal type 4 | Mm.25246 | AV066321 | 99.5 | 1.18E-04 |
1418550_x_at | Defcr-rs1 | defensin related sequence cryptdin peptide (paneth cells) | Mm.14269 | NM_007844 | 22.8 | 0.007 |
1449478_at | Mmp7 | matrix metalloproteinase 7 | Mm.4825 | NM_010810 | 9.0 | 0.002 |
1434852_at | Pla2g2e | phospholipase A2, group IIE | Mm.89936 | AV228827 | 8.0 | 0.049 |
1427873_at | Defcr15 | defensin related cryptdin 15 | Mm.195047 | U03065 | 3.4 | 0.019 |
1419254_at | Mthfd2 | methylenetetrahydrofolate dehydrogenase (NAD+ dependent) | Mm.443 | BG076333 | 2.6 | 0.004 |
Other cells | ||||||
1417459_at | Dcpp | demilune cell and parotid protein | Mm.193062 | NM_019910 | 285.6 | 8.59E-05 |
1450618_a_at | Sprr2a | small proline-rich protein 2A | Mm.6853 | NM_011468 | 34.2 | 2.39E-04 |
1449279_at | Gpx2 | glutathione peroxidase 2 | Mm.57225 | NM_030677 | 18.6 | 1.83E-04 |
1418931_at | Reg4 | regenerating islet-derived family member 4 | Mm.46306 | NM_026328 | 15.4 | 0.001 |
1417370_at | Tff3 | trefoil factor 3, intestinal | Mm.4641 | NM_011575 | 12.0 | 0.001 |
1419127_at | Npy | neuropeptide Y | Mm.154796 | NM_023456 | 7.2 | 0.033 |
1427355_at | Calca | calcitonin/calcitonin-related polypeptide, alpha | Mm.4361 | X97991 | 5.0 | 0.019 |
1420965_a_at | Enc1 | ectodermal-neural cortex 1 | Mm.241073 | BM120053 | 4.1 | 6.25E-04 |
1448290_at | Pap | pancreatitis-associated protein | Mm.2553 | NM_011036 | 2.6 | 0.022 |
1417281_a_at | Mmp23 | matrix metalloproteinase 23 | Mm.29373 | NM_011985 | 2.4 | 0.045 |
1422597_at | Mmp15 | matrix metalloproteinase 15 | Mm.7283 | NM_008609 | 2.4 | 0.003 |
1421195_at | Cckar | cholecystokinin A receptor | Mm.3521 | BC020534 | 2.1 | 0.004 |
Extracellular signaling factors | ||||||
1416211_a_at | Ptn | pleiotrophin | Mm.3063 | BF178348 | 25.5 | 7.50E-05 |
1454159_a_at | Igfbp2 | insulin-like growth factor binding protein 2 | Mm.141936 | AK011784 | 24.9 | 0.007 |
1422324_a_at | Pthlh | parathyroid hormone-like peptide | Mm.28440 | NM_008970 | 22.8 | 0.001 |
1422300_at | Nog | noggin | Mm.39094 | NM_008711 | 12.8 | 9.64E-04 |
1423635_at | Bmp2 | Bmp2 | Mm.29877 | AV239587 | 9.3 | 0.019 |
1448254_at | Ptn | pleiotrophin | Mm.3063 | BC002064 | 7.9 | 7.62E-04 |
1418910_at | Bmp7 | bone morphogenetic protein 7 | Mm.595 | NM_007557 | 6.2 | 0.004 |
1450922_a_at | Tgfb2 | transforming growth factor, beta 2 | Mm.18213 | AW049938 | 4.5 | 1.06E-04 |
1420518_a_at | Igsf9 | immunoglobulin superfamily, member 9 | Mm.214530 | AF317839 | 4.4 | 0.007 |
1450923_at | Tgfb2 | transforming growth factor, beta 2 | Mm.18213 | BF144658 | 4.3 | 7.04E-04 |
1416006_at | Mdk | midkine | Mm.906 | M34328 | 4.1 | 4.19E-04 |