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Table 1 Selected genes up- or down-regulated in transgenic lungs

From: Hyperactive Wnt signaling changes the developmental potential of embryonic lung endoderm

Affymetrix probe ID Allele Gene name GenBank accession numbers   Fold change (transgenic/ wild-type) p value
Genes down-regulated in transgenic lungs
Specialized cell markers
1418818_at Aqp5 aquaporin 5 Mm.45580 NM_009701 96.1 0.009
1449015_at Retnla resistin like alpha Mm.33772 NM_020509 37.5 0.004
1436996_x_at Lyzs lysozyme Mm.45436 AV066625 36.5 6.82E-04
1451537_at Chi3l1 chitinase 3-like 1 Mm.4376 BC005611 30.5 0.002
1429626_at Sftpa surfactant associated protein A Mm.46062 AV024301 30.1 0.004
1420504_at Slc6a14 solute carrier family 6 (neurotransmitter transporter)14 Mm.25770 AF320226 21.4 0.023
1419020_at Gif gastric intrinsic factor Mm.456 NM_008118 19.7 0.003
1416456_a_at Chia chitinase, acidic Mm.46418 BC011134 16.0 0.012
1423547_at Lyzs lysozyme Mm.45436 AW208566 14.8 0.004
1419764_at Chi3l3 chitinase 3-like 3 Mm.4571 NM_009892 13.6 0.013
1420378_at Sftpd surfactant associated protein D Mm.1321 BC003705 12.0 2.10E-04
1448553_at Myh7 myosin, heavy polypeptide 7, cardiac muscle, beta Mm.155714 NM_080728 11.7 0.011
1455431_at Slc5a1 solute carrier family 5 (sodium/glucose cotransporter)1 Mm.25237 AV371434 10.4 0.025
1437028_at Sftpb surfactant associated protein B Mm.46033 AV025094 9.8 5.41E-04
1438696_at Edn3 endothelin 3 Mm.9478 BB368452 7.0 0.016
1452543_a_at Scgb1a1 secretoglobin, family 1A, member 1 (uteroglobin) Mm.2258 X67702 5.2 0.014
1425291_at Foxj1 forkhead box J1 Mm.4985 L13204 2.3 0.006
Genes up-regulated in transgenic lungs
Cell proliferation markers
1451417_at Brca1 breast cancer 1 Mm.1889 U31625 9.5 0.004
1416969_at Gtse1 G two S phase expressed protein 1 Mm.20858 NM_013882 5.9 7.58E-04
1416122_at Ccnd2 cyclin D2 Mm.3141 NM_009829 3.5 1.27E-04
1422460_at Mad2l1 MAD2 (mitotic arrest deficient, homolog)-like 1 (yeast) Mm.43444 NM_019499 3.2 0.014
1425166_at Rbl1 retinoblastoma-like 1 (p107) Mm.2994 U27178 3.1 0.007
1417420_at Ccnd1 cyclin D1 Mm.22288 BB538325 2.2 0.002
Transcription factors and Notch/Delta signaling
1421299_a_at Lef1 lymphoid enhancer binding factor 1 Mm.200634 NM_010703 58.7 1.29E-04
1426552_a_at Bcl11a B-cell CLL/lymphoma 11A (zinc finger protein) Mm.24020 BB772866 19.5 0.001
1417679_at Gfi1 growth factor independent 1 Mm.2078 NM_010278 18.0 0.038
1449236_at Dll3 delta-like 3 (Drosophila) Mm.12896 AB013440 16.7 0.001
1424903_at Smcy selected mouse cDNA on the Y Mm.1064 AF127244 10.8 0.006
1451835_at Sox21 SRY-box containing gene 21 Mm.70950 AY069926 8.9 0.001
1449822_at Atoh1 atonal homolog 1 (Drosophila) Mm.57229 BC010820 7.4 0.001
1422914_at Sp5 transacting transcription factor 5 Mm.155690 NM_022435 7.1 0.043
1448595_a_at Rex3 reduced expression 3 Mm.14768 NM_009052 6.5 2.55E-04
1450164_at Ascl1 achaete-scute complex homolog-like 1 (Drosophila) Mm.10663 NM_008553 6.1 0.007
1460336_at Ppargc1 peroxisome proliferative activated receptor, gamma, coactivator 1 Mm.10707 BB745167 5.7 0.039
1418055_at Neurod4 neurogenic differentiation 4 Mm.10695 NM_007501 5.2 0.017
1424950_at Sox9 SRY-box containing gene 9 Mm.46607 BI077717 5.1 0.019
1450339_a_at Bcl11b B-cell lymphoma/leukaemia 11B Mm.116831 NM_021399 5.0 0.042
1460214_at Pcp4 Purkinje cell protein 4 Mm.5023 NM_008791 4.5 0.012
1415811_at Np95 nuclear protein 95 Mm.42196 BB702754 4.0 0.037
1419437_at Sim2 single-minded 2 Mm.4775 NM_011377 3.9 0.03
1415810_at Np95 nuclear protein 95 Mm.42196 NM_010931 3.9 0.017
1451255_at Lisch7 liver-specific bHLH-Zip transcription factor Mm.4067 BC004672 3.7 8.53E-04
1421951_at Lhx1 LIM homeobox protein 1 Mm.4965 NM_008498 3.3 0.014
1417302_at Rcor RE1-silencing transcription factor (REST) co-repressor Mm.22980 NM_054048 3.3 0.047
1422088_at Lmyc1 Mus musculus, clone IMAGE:1528432, mRNA Mm.198846 BI687857 3.0 0.001
1424942_a_at Myc myelocytomatosis oncogene Mm.2444 BC006728 2.6 7.85E-04
1417155_at Nmyc1 neuroblastoma myc-related oncogene 1 Mm.16469 BC005453 2.3 0.01
Specialized cell markers
Paneth cells
1450709_at Defcr5 defensin related cryptdin 5 Mm.140173 NM_007851 844.0 0.003
1416905_at Guca2 guanylate cyclase activator 2 (guanylin 2, intestinal, heatstable) Mm.2614 NM_008190 322.2 0.001
1450631_x_at Defcr6 defensin related cryptdin 6 Mm.246485 NM_007852 187.6 0.002
1427119_at Spink4 serine protease inhibitor, Kazal type 4 Mm.25246 AV066321 99.5 1.18E-04
1418550_x_at Defcr-rs1 defensin related sequence cryptdin peptide (paneth cells) Mm.14269 NM_007844 22.8 0.007
1449478_at Mmp7 matrix metalloproteinase 7 Mm.4825 NM_010810 9.0 0.002
1434852_at Pla2g2e phospholipase A2, group IIE Mm.89936 AV228827 8.0 0.049
1427873_at Defcr15 defensin related cryptdin 15 Mm.195047 U03065 3.4 0.019
1419254_at Mthfd2 methylenetetrahydrofolate dehydrogenase (NAD+ dependent) Mm.443 BG076333 2.6 0.004
Other cells
1417459_at Dcpp demilune cell and parotid protein Mm.193062 NM_019910 285.6 8.59E-05
1450618_a_at Sprr2a small proline-rich protein 2A Mm.6853 NM_011468 34.2 2.39E-04
1449279_at Gpx2 glutathione peroxidase 2 Mm.57225 NM_030677 18.6 1.83E-04
1418931_at Reg4 regenerating islet-derived family member 4 Mm.46306 NM_026328 15.4 0.001
1417370_at Tff3 trefoil factor 3, intestinal Mm.4641 NM_011575 12.0 0.001
1419127_at Npy neuropeptide Y Mm.154796 NM_023456 7.2 0.033
1427355_at Calca calcitonin/calcitonin-related polypeptide, alpha Mm.4361 X97991 5.0 0.019
1420965_a_at Enc1 ectodermal-neural cortex 1 Mm.241073 BM120053 4.1 6.25E-04
1448290_at Pap pancreatitis-associated protein Mm.2553 NM_011036 2.6 0.022
1417281_a_at Mmp23 matrix metalloproteinase 23 Mm.29373 NM_011985 2.4 0.045
1422597_at Mmp15 matrix metalloproteinase 15 Mm.7283 NM_008609 2.4 0.003
1421195_at Cckar cholecystokinin A receptor Mm.3521 BC020534 2.1 0.004
Extracellular signaling factors
1416211_a_at Ptn pleiotrophin Mm.3063 BF178348 25.5 7.50E-05
1454159_a_at Igfbp2 insulin-like growth factor binding protein 2 Mm.141936 AK011784 24.9 0.007
1422324_a_at Pthlh parathyroid hormone-like peptide Mm.28440 NM_008970 22.8 0.001
1422300_at Nog noggin Mm.39094 NM_008711 12.8 9.64E-04
1423635_at Bmp2 Bmp2 Mm.29877 AV239587 9.3 0.019
1448254_at Ptn pleiotrophin Mm.3063 BC002064 7.9 7.62E-04
1418910_at Bmp7 bone morphogenetic protein 7 Mm.595 NM_007557 6.2 0.004
1450922_a_at Tgfb2 transforming growth factor, beta 2 Mm.18213 AW049938 4.5 1.06E-04
1420518_a_at Igsf9 immunoglobulin superfamily, member 9 Mm.214530 AF317839 4.4 0.007
1450923_at Tgfb2 transforming growth factor, beta 2 Mm.18213 BF144658 4.3 7.04E-04
1416006_at Mdk midkine Mm.906 M34328 4.1 4.19E-04
  1. For details of the Affymetrix GeneChip mouse 430A array analysis, which used RNA from three transgenic and three wild-type E18.5 lungs, see Materials and methods. Only selected genes taken from categories discussed in the text are shown. For the complete set of genes up- or down-regulated more than two-fold, and for the raw data, see Additional data files 2, 3 and 4.