Figure 4

UNC-69::GFP is expressed in neurons. Confocal micrographs of mosaic animals expressing a rescuing carboxy-terminal UNC-69::GFP fusion. A 1 μm optical section is shown in (a) all other panels are projections of optical series. (a) Late gastrula (large arrowhead) and early comma-stage embryo (arrow) with widespread expression of UNC-69::GFP. Embryos were still inside the mother. Small arrowheads indicate the maternal VNC; v indicates the maternal vulva. (b) A two-fold-stage embryo with strong UNC-69::GFP expression in VNC neurons (between arrowheads). (c) A three-fold embryo expressing UNC-69::GFP in a growth cone (arrowhead). The arrow indicates a neuronal cell body. (d) An L1-stage larva expressing UNC-69::GFP in neurons and axons in the head (arrow), VNC (small arrowheads) and tail (large arrowhead). The asterisk indicates reporter expression in labial sensory neuronal processes of an adjoining adult animal. (e) An L3 larva expressing UNC-69::GFP in the CAN neuron (large arrow), excretory canal (small arrowheads) and in commissural axons (small arrow). (f) An L4 larva expressing UNC-69::GFP in the CAN (large arrow), HSN (large arrowhead) and ALM (small arrowhead) neurons. Small arrows indicate commissures. All scale bars represent 10 μm. In all cases, anterior is to the left and dorsal is up.