UNC-69 and UNC-76 cooperate to regulate the size and position of synaptic vesicles. (a-d) Lateral view of adult hermaphrodites 52–54 h after hatching, single section. (e,f) Lateral view of the DNC of adult hermaphrodites 52–54 h after hatching, flattened images of confocal z-stack. Anterior is to the left and dorsal is up. (a,e) SNB-1::GFP is evenly distributed along the DNC in wild-type animals. (b) Removing one copy of unc-69 does not affect SNB-1::GFP distribution. (c,d,f) SNB-1::GFP becomes diffused and the puncta becomes larger (arrows) in unc-69(e587)/+; unc-76(e911)/+ and (unc-69(e587)/+; unc-76(n2457)/+ double heterozygotes. Occasionally, SNB-1::GFP is missing altogether from a stretch of the DNC (bracket in (d)). The genotypes are as follows: (a,e) juIs1 [Punc-25::snb-1::gfp], (b) qC1/unc-69(e587); juIs1, (c) qC1/unc-69(e587); nT1[qIs51]/juIs1; nT1[qIs51]/unc-76(e911), (d,f) qC1/unc-69(e587); nT1[qIs51]/juIs1; nT1[qIs51]/unc-76(n2457). Scale bars represent 10 μm.