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Table 1 Axon outgrowth and guidance defects in unc-69 mutants

From: The short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans

  ALM defect (%)   
Genotype B NR E FL   n
Wild type 0 0 0 12   113
unc-69(e602) 12 36 77 84   77
unc-69(e587) 15 45 85 89   80
unc-69(e602) (m+z-) 0 4.3 39 91   70
unc-69(e602)/nDf40 (m+z-) 0 7.2 20 72   69
unc-69(e587) (m+z-) 1.4 7.2 43 87   69
unc-69(e587)/nDf40 (m+z-) 0 8.3 20 82   60
unc-69(e602)* 19 48 62 95   113
unc-69(e602); opEx [P mec-7 ::unc-69]* 1 12 2 5   81
unc-69(e602); opEx [P mec-7 ::unc-69]* 5 9 6 10   79
unc-69(e602); opEx [P mec-7 ::unc-69]* 8 29 0 10   85
  AVM defect (%)  
Genotype B NR E FL V n
Wild type 0 0 1 8 0 106
unc-69(e602) 32 27 72 73 2.7 77
unc-69(e587) 64 70 86 87 0 80
unc-69(e602) (m+z-) 1.4 4.3 57 86 0 70
unc-69(e602)/nDf40 (m+z-) 0 0 56 80 0 69
unc-69(e587) (m+z-) 2.9 8.8 56 85 0 69
unc-69(e587)/nDf40 (m+z-) 0 3.6 76 95 0 60
unc-69(e602)* 46 67 93 100 ND 113
unc-69(e602); opEx [P mec-7 ::unc-69]* 0 4 4 4 ND 81
unc-69(e602); opEx [P mec-7 ::unc-69]* 8 12 12 23 ND 79
unc-69(e602); opEx [P mec-7 ::unc-69]* 11 21 12 13 ND 85
  1. Neurite outgrowth and guidance defects of mechanosensory touch neurons in unc-69 mutants. The morphology of neurites of ALM (top) and AVM (bottom) neurons (as in the schematic in Figure 2) was scored in different unc-69 mutants, in unc-69/nDf40 heterozygotes, and in mosaic animals carrying a functional unc-69 transgene under the control of the mec-7 promoter, which directs expression in the six touch neurons. All worms scored had a Pmec-4::gfp transgene zdIs5 in the background to allow visualization of the neurite morphology. One ALM neurite was scored per animal. B, failure to form proper branch at the nerve ring; NR, failure of nerve ring branch to fully extend; E: failure to elongate past the branch point; FL, failure to extend fully; V, ventral guidance defect. (m+z-): homozygous mutant animals derived from heterozygous mothers. *These strains also carry a lin-15(n765) mutation in the background. All opEx transgenes also carry a wild-type copy of lin-15(+) as a coinjection marker. ND, not done. n, number of worms scored.