The short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans

Table 1 Axon outgrowth and guidance defects in unc-69 mutants

Neurite outgrowth and guidance defects of mechanosensory touch neurons in unc-69 mutants. The morphology of neurites of ALM (top) and AVM (bottom) neurons (as in the schematic in Figure 2) was scored in different unc-69 mutants, in unc-69/nDf40 heterozygotes, and in mosaic animals carrying a functional unc-69 transgene under the control of the mec-7 promoter, which directs expression in the six touch neurons. All worms scored had a P_mec-4::gfp transgene zdIs5 in the background to allow visualization of the neurite morphology. One ALM neurite was scored per animal. B, failure to form proper branch at the nerve ring; NR, failure of nerve ring branch to fully extend; E: failure to elongate past the branch point; FL, failure to extend fully; V, ventral guidance defect. (m+z-): homozygous mutant animals derived from heterozygous mothers. *These strains also carry a lin-15(n765) mutation in the background. All opEx transgenes also carry a wild-type copy of lin-15(+) as a coinjection marker. ND, not done. n, number of worms scored.