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Table 3 Quantitative analysis of axon extension defects in unc-69(lf), unc-76(lf) and other mutants

From: The short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans

Genotype 2 AWCOFF (%) 1 AWCON (%) 2 AWCON (%) n
Wild type 1 99 0 442
unc-69     
   unc-69(ju69) 1 99 0 190
   unc-69(e602) 34 66 0 119
   unc-69(e587) 30 70 0 194
unc-76     
   unc-76(rh116) 11 89 0 83
   unc-76(n2457) 6 94 0 102
   unc-76(n2397) 8 92 0 64
   unc-76(ev424) 10 90 0 68
   unc-76(n2367) 30 70 0 84
   unc-76(n2399) 25 75 0 67
   unc-76(e911) 47 53 0 101
   unc-76(e911); lon-2(e678) 31 69 0 91
   unc-76(n2398) 28 72 0 184
Double mutants     
   unc-69(e602); unc-76(n2457) 35 65 0 106
   unc-69(e602); unc-76(e911) 48 52 0 118
   unc-69(e587); unc-76(n2457) 33 67 0 108
   unc-69(e587); unc-76(e911) 31 69 0 143
Other axonal guidance mutants     
   sax-3(ky123) 64 33 3 112
   lon-2(e678) unc-6(n102) 38 62 0 65
   vab-3(e648) 54 40 6 68
   unc-33(e204) 7 73 20 135
   unc-119(ed3) 12 48 39 99
Other double mutants     
   unc-76(e911); sax-3(ky123) 95 5 0 22
   unc-76(e911); lon-2(e678) unc-6(n102) 73 27 0 152
   unc-76(e911); vab-3(e648) 63 27 10 62
   unc-33(e204); unc-76(e911) 80 18 1 291
   unc-119(ed3); unc-76(e911) 65 32 3 167
  1. All animals scored had kyIs140 (Pstr-2::gfp) in the background, which turns on its expression in only one of the two AWC neurons (1 AWCON) in wild-type animals. In axon guidance mutants, Pstr-2::gfp expression is silenced in both AWCs (2 AWCOFF) owing to failure of axonal contact. All unc-69 and unc-76 alleles except unc-76(rh116) are arranged in order according to their physical position (5' to 3') in the open reading frame. n, number of animals scored.