Figure 6

Cell replication and protein renewal in post-ischemic mouse kidney analyzed with double labeling with BrdU, analyzed as ⁸¹Br- and ¹⁵N-leucine. (a,b) Wide-view parallel quantitative mass image of (a) ¹²C¹⁴N^- and (b) ⁸¹Br^-. The ⁸¹Br^- label indicates a cell with replicated DNA. Field: 100 μm × 100 μm (256 × 256 pixels); acquisition time 2 min. (c-e) Higher-resolution parallel images of the boxed regions in (a,b) for (c) ¹²C¹⁴N^-; (d) ³¹P^-; (e) ⁸¹Br^-. The ³¹P^- image enables identification of other cells with unreplicated DNA. Field: 23 × 23 μm (256 × 256 pixels); acquisition time 60 min. (f,g) Parallel quantitative mass images for (f) ¹²C¹⁴N^- and (g) ¹²C¹⁵N^-, from which protein renewal is calculated. Field: 23 × 23 μm (256 × 256 pixels); acquisition time 10 min. (h) Quantitation of protein renewal in replicating and non-replicating cells. Cy, cytoplasm; NQ, nucleus of non-replicating cells; NR, nucleus of replicating cells.