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Table 1 BCNU affects different neural progenitor cell populations equally in vivo

From: CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo

Cell population SVZ control SVZ BCNU DG control DG BCNU CC control CC BCNU
DCX+ 38 ± 5 43 ± 5 70 ± 6 60 ± 14 ND ND
Olig2+ 14 ± 7 13 ± 13 15 ± 2 15 ± 3 86 ± 2 86 ± 11
S-100β+ 2 ± 4 2 ± 3 10 ± 6 2 ± 3 10 ± 6 14 ± 11
  1. In these experiments, BrdU-labeled cells were co-analyzed for expression of cell-type specific antigens by confocal microscopy, as in Figures 5–7, for the same animals as were analyzed for Figure 5. All cells were analyzed by z-stack analysis to confirm identity of the BrdU+ incorporation and labeling with cell-type specific antibodies. Numbers are provided as average percentages ± SEM of all BrdU+ cells identified for each animal, as described in Materials and methods. DCX expression was not analyzed (ND) in the corpus callosum (CC), because of the lack of neuronal progenitor cells in white-matter tracts. The data show that, despite the reduction in total numbers of BrdU+ cells in each tissue, each individual cell population was affected similarly, and did not change in its proportional contribution to the entire population of BrdU+ cells. The only possible exception to this is the representation of BrdU+ GFAP+ cells in the dentate gyrus (DG), but the difference between this set and controls did not achieve significance. SVZ, subventricular zone.