Table 3 Comparison of the analysis of gene expression by quantitative RT-PCR and microarray
From: Dosage compensation is less effective in birds than in mammals
| Â | Â | M:F (microarray) | M:F (RT-PCR) | ||
|---|---|---|---|---|---|
Species and tissue | Affymetrix number or gene symbol | Ratio | P-value | Ratio | P-value |
Zebra finch | Â | Â | Â | Â | Â |
Adult brain | FST | 1.7 | 4.0E-04 | 1.8 | 5.0E-04 |
| Â | LUZP1 | 1.6 | 3.7E-03 | 2.4 | 1.0E-06 |
| Â | SMARCA2 | 1.4 | 1.7E-03 | 2.1 | 2.0E-06 |
| Â | DNAJA1 | 1.3 | 1.6E-03 | 2.0 | 4.6E-05 |
| Â | CRHBP | 1.2 | 5.9E-02 | 1.4 | 2.8E-03 |
| Â | RPS6 | 1.6 | 2.4E-05 | 2.1 | 1.3E-04 |
P1 brain | FST | 1.6 | 1.5E-02 | 1.5 | 3.0E-02 |
| Â | LUZP1 | 1.7 | 1.8E-02 | 2.5 | 4.4E-04 |
| Â | SMARCA2 | 1.2 | 1.7E-01 | 1.8 | 9.0E-04 |
| Â | DNAJA1 | 1.1 | 2.1E-02 | 2.1 | 1.3E-05 |
| Â | CRHBP | ND | ND | 2.1 | 8.7E-04 |
| Â | RPS6 | 1.6 | 2.4E-04 | 2.5 | 3.0E-06 |
Chicken | Â | Â | Â | Â | Â |
E14 brain | Gga.12454.1.S1 | 0.4 | 4.9E-05 | 0.5 | 7.3E-05 |
| Â | GgaAffx.9524 | 1.0 | 0.59 | 1.1 | 0.64 |
| Â | GgaAffx.24493 | 1.6 | 5.0E-06 | 1.8 | 1.3E-03 |
| Â | Gga.4811 | 1.8 | 9.9E-08 | 2.3 | 2.5E-04 |
| Â | GgaAffx.25289 | 2.0 | 1.7E-06 | 2.7 | 9.0E-05 |
| Â | Gga.2433 | 2.4 | 1.1E-07 | 2.6 | 2.9E-04 |
| Â | Gga.2883 | 2.7 | 1.3E-08 | 2.6 | 1.2E-05 |