A global analysis of genetic interactions in Caenorhabditis elegans

Journal of Biology

Table 1 A summary of the query genes

Query gene	Ortholog (pathway)	Null/strong loss-of-function phenotype(s)	Hypomorphic phenotype(s)	References
let-756	FGF (FGF)	Early larval arrest (s2887)	scrawny, Slo (s2613)**	[77]
egl-15	FGF receptor (FGF)	Early larval arrest (n1456)	scrawny, Egl (n1477)**	[78]
let-23	EGF receptor (EGF)	L1 arrest (mn23)	ts Vul, pleotropic (n1045)**	[79]
daf-2	Insulin growth factor receptor (insulin)	Emb (e979)	ts Daf-c (e1370)**	[35]
sem-5	GRB-2 (EGF, FGF, insulin)	L1 arrest (leaky) (n1619)	Egl, Vul (n2019)*	[79,80]
sos-1	Guanine-nucleotide exchange factor (EGF, FGF)	Emb (s1031)	ts Egl, Vul (cs41)*	[33]
let-60	RAS (EGF, FGF, insulin, Wingless/Wnt)	Mid-larval lethal (leaky) (s1124)	Egl, Vul (n2021)*	[81,82]
glp-1	Notch receptor (Notch)	ts Emb (gp60)	ts Emb, Glp, Muv (or178)*	[47]
bar-1	β-catenin (Wingless (Wnt))	Mig, Vul, Pvl (ga80)**	Mig, Vul, Pvl (mu63)	[34]
sma-6	Type I TGF-β receptor (TGF-β)	Sma, Mab (wk7)	Sma (e1482)*	[83]
clk-2	Tel-2p (DNA-damage response)	Unknown	Slo, Ste, ts Emb (mn159)**	[84]

In the second column, 'ortholog' refers to the canonical ortholog in yeast, flies, mice, or humans. The pathway to which the ortholog belongs is in brackets. Third column: if known, the null or strong loss-of-function phenotype is shown. Fourth column: weak loss-of-function (hypomorphic) phenotypes are shown for representative alleles. Phenotypic acronyms: Emb, embryonic lethal; Daf-c, dauer formation constitutive; Slo, slow growth; Egl, egg-laying defective; Vul, vulvaless; Glp, germ-line proliferation defects; Muv, multivulva; Mig, cell and/or axon migration defects; Pvl, protruding vulva; Sma, small body; Mab, male tail abnormal; Ste, sterile; ts, temperature sensitive. The alleles used in this study are followed by two asterisks if used as a query against both the signaling targets and the LGIII targets, or just a single asterisk if used only against the signaling targets.