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Figure 2 | Journal of Biology

Figure 2

From: Imp-L2, a putative homolog of vertebrate IGF-binding protein 7, counteracts insulin signaling in Drosophila and is essential for starvation resistance

Figure 2

Imp-L2 controls body and organ size. (a) Tangential section through an adult eye containing an Imp-L2 overexpression clone marked by the lack of red pigment. Within the clone, the size of the ommatidia is reduced. Wild-type ommatidia close to the clone are also smaller (compare black circled areas). (b) Eye-specific overexpression of UAS-s.Imp-L2 reduces male body weight (-38.3%, P = 7 × 10-42). (c) Overexpression of UAS-Imp-L2 by ppl-Gal4 results in a 56.1% weight reduction in male flies, whereas ppl-Gal4 driven expression of UAS-s.Imp-L2 results in lethality (†). P = 3 × 10-47. (d) Loss of Imp-L2 function increases body size in males (top) and females (bottom). (e) Analyses of male and female weights. Wing area, cell number and cell size were assessed in female adult wings. GR indicates Imp-L2 genomic rescue construct. P-values are indicated by numbers as follows: 1, 2 × 10-33; 2, 8 × 10-18; 3, 9 × 10-16; 4, 6 × 10-7; 5, 3 × 10-46; 6, 1 × 10-24; 7, 8 × 10-31; 8, 2 × 10-7; 9, 4 × 10-4; 10, 4 × 10-7; 11, 3 × 10-7; 12, 1 × 10-4. Genotypes: 'control' y, w/w; 'Imp-L2-/-' y, w; Imp-L2Def42/Imp-L2Def20; 'Imp-L2+/-' for the weight analysis (e) y, w; Imp-L2 Def20/+; 'Imp-L2+/-' for the wing analysis (e) y, w; Imp-L2 Def42/+; 'Imp-L2-/-, GR' y, w; Imp-L2 Def42/Imp-L2 Def20, GR-57; 'Imp-L2+/-, GR' y, w; Imp-L2 Def20, GR-57/+. P-values were determined using unpaired Student's t-test against the control except in (4) where the weight of IMP-L2+/- was compared to IMP-L2+/-, GR. n = 40 for the weight analysis in (b,c,e); n = 12 for the wing analysis in (e). Error bars represent s.d.

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