Systematic identification of regulatory proteins critical for T-cell activation
- Peter Chu†1,
- Jorge Pardo†1,
- Haoran Zhao†1,
- Connie C Li1, 4,
- Erlina Pali1,
- Mary M Shen1,
- Kunbin Qu1,
- Simon X Yu1,
- Betty CB Huang1,
- Peiwen Yu1, 4,
- Esteban S Masuda1,
- Susan M Molineaux1,
- Frank Kolbinger2,
- Gregorio Aversa3,
- Jan de Vries3,
- Donald G Payan1Email author and
- X Charlene Liao1, 5Email author
© Chu et al., licensee BioMed Central Ltd. 2003
Received: 19 August 2002
Accepted: 7 August 2003
Published: 15 September 2003
The activation of T cells, mediated by the T-cell receptor (TCR), activates a battery of specific membrane-associated, cytosolic and nuclear proteins. Identifying the signaling proteins downstream of TCR activation will help us to understand the regulation of immune responses and will contribute to developing therapeutic agents that target immune regulation.
In an effort to identify novel signaling molecules specific for T-cell activation we undertook a large-scale dominant effector genetic screen using retroviral technology. We cloned and characterized 33 distinct genes from over 2,800 clones obtained in a screen of 7 × 108 Jurkat T cells on the basis of a reduction in TCR-activation-induced CD69 expression after expressing retrovirally derived cDNA libraries. We identified known signaling molecules such as Lck, ZAP70, Syk, PLCγ1 and SHP-1 (PTP1C) as truncation mutants with dominant-negative or constitutively active functions. We also discovered molecules not previously known to have functions in this pathway, including a novel protein with a RING domain (found in a class of ubiquitin ligases; we call this protein TRAC-1), transmembrane molecules (EDG1, IL-10Rα and integrin α2), cytoplasmic enzymes and adaptors (PAK2, A-Raf-1, TCPTP, Grb7, SH2-B and GG2-1), and cytoskeletal molecules (moesin and vimentin). Furthermore, using truncated Lck, PLCγ1, EDG1 and PAK2 mutants as examples, we showed that these dominant immune-regulatory molecules interfere with IL-2 production in human primary lymphocytes.
This study identified important signal regulators in T-cell activation. It also demonstrated a highly efficient strategy for discovering many components of signal transduction pathways and validating them in physiological settings.
Activation of specific signaling pathways in lymphocytes determines the quality, magnitude and duration of immune responses. These pathways are also responsible for the induction, maintenance and exacerbation of physiological or pathological lymphocyte responses in transplantation, acute and chronic inflammatory diseases, and autoimmunity. The activation of T lymphocytes is triggered when the T-cell receptor (TCR) recognizes antigens presented by the major histocompatibility complex (MHC) in antigen-presenting cells . Engagement of the TCR by antigen-MHC results in rearrangement of the actin cytoskeleton, induction of gene transcription, and progression into the cell cycle [2, 3]. The proximal events of TCR signaling include activation of the Src-family kinases Lck and Fyn, phosphorylation of TCR components, and activation of ZAP70 and Syk tyrosine kinases, as well as recruitment of adaptor molecules (LAT and SLP-76), which in turn couple to more distal signaling pathways including Ras and PLCγ [4, 5]. Using classical genetic and biochemical approaches, new components of the TCR signaling pathway are being discovered, albeit at a slow pace. Efficient identification of additional signaling molecules probably requires novel approaches.
Here, we describe our attempt to identify and validate novel signaling molecules specific for T-cell activation. We used up-regulation of the cell-surface marker CD69 in T cells to monitor TCR activation; CD69 as an activation marker has been well validated , more recently using T cells deficient in certain key signaling molecules such as SLP-76 and LAT [7, 8]. The rationale of this 'functional genomics' screen was to identify cell clones whose CD69 upregulation was repressed following introduction of clones from a retroviral cDNA library. The library clones conferring such repression would then represent immune modulators that function to block TCR signal transduction.
Jurkat Clone 4D9 was selected for low basal levels of CD69 expression and strong induction following TCR stimulation (see Additional data file 1 for details of the selection and infection procedures). The 'Tet-off' system was adapted for regulated expression of the retroviral cDNA library: cDNA inserts in the retroviral library were cloned behind the tetracycline (Tet) regulatory element (TRE) and the cytomegalovirus (CMV) minimal promoter. Transcription of the cDNA inserts was then dependent on the presence of tetracycline-controlled transactivator (tTA) , a fusion of Tet repression protein and the VP16 activation domain, and the absence of tetracycline or its derivatives such as doxycycline (Dox). A derivative of Jurkat clone 4D9 stably expressing tTA, called 4D9#32, was engineered and selected (see Additional data file 1).
Screening for cells lacking CD69 upregulation
Given our experimental design, we expected the expression of retroviral cDNAs and their putative inhibitory effect to be turned off with the addition of Dox. This feature helped us to ascertain that the phenotype was due to expression of the cDNA library rather than to epigenetic changes or spontaneous or retroviral-insertion-mediated somatic mutation(s). To confirm this, we compared anti-TCR-induced CD69 expression in the presence and absence of Dox. As shown in Figure 2c, cells with the CD69lowCD3+ phenotype decreased from 24.0% to 13.0% with the addition of Dox, demonstrating that a significant number of cells (11%) had lost the CD69lowCD3+ phenotype when library-cDNA expression was turned off. These data suggested that the CD69lowCD3+ phenotype in a significant proportion (at least 11% out of 24%, or 45.8%) of cells in this population was indeed caused by expression of the cDNA-library clones.
Functional analysis of single-cell clones
Overview of identified molecular targets
Known to function in TCR pathway
Relative to ORF*
-147, +787 nt
-21, +809 nt
-27, +1012 nt
-59, +799 nt
+1409, +2282 nt
+472, >+2021 nt
-55, +1285 nt
-237, +644 nt
-136, +479 nt
Enzymes and receptors
-58, +1108 nt
p21-activated kinase 2
-50, +339 nt
p21-activated kinase 2
-42, +670 nt
-4, +456 nt
<-244, +942 nt
<-244, +1037 nt
RING finger ubiquitin ligase
-254, +510 nt
+689, +1350 nt
+3348, +3914 nt
+703, +1374 nt
+817, +1112 nt
Adaptors and transcription factors
+1268, +1912 nt
-97, +1795 nt
+1352, +1960 nt
+914, +1202 nt
-93, +413 nt
+1026, +2069 nt
Novel (130 amino acids; no homology)
Novel signaling molecule
+1, +121 nt
Secretory carrier membrane protein
-5, +833 nt
C2 domain (Ca2+- or IP-binding)
EST from clone 2108068
LPP20 lipoprotein precursor
-22, +713 nt
-93, +1534 nt
-98, +374 nt
CpG island? (clone 550H1)
IgG2 heavy chain
Ig light chain
Characterization of proteins critical for T-cell activation
As shown in Table 1, we obtained known TCR regulators such as Lck, ZAP70, Syk, PLCγ1, PAG, SHP-1/PTP1C, Csk and nucleolin (reviewed in ). The hits with the highest frequency, however, were those encoding the TCR β subunit. This new β chain leads to the assembly of a new TCR complex no longer recognizable by the stimulating antibody C305, because C305 only recognizes the original endogenous Jurkat clonotypic TCR complex  (see also Additional data file 5).
TCR engagement leads to rapid tyrosine phosphorylation and activation of PLCγ1 . One of our hits contained the pleckstrin homology (PH) domain and the amino- and carboxy-terminal SH2 domains of PLCγ1 (Figure 4c). Significantly, this hit also lacked the crucial tyrosine Y783, which is essential for coupling of TCR stimulation to IL-2 promoter activation. The Y783F mutant is a very potent dominant-negative form of PLCγ1 . Indeed, the original clone encoding the PLCγ1 hit had the highest Dox ratio for CD69 expression among all clones analyzed. When introduced into naïve Jurkat cells, this fragment also caused a severe block of TCR-induced CD69 expression (Figure 4c).
In addition to known signaling molecules, we also discovered genes whose sequences had been reported previously but whose involvement in TCR signaling was not documented (Table 1, and see Additional data files 6 and 7). EDG1 (endothelial differentiation gene-1) was discovered initially from a set of immediate-early-response gene products cloned from human umbilical vein endothelial cells . EDG1 is a G-protein-coupled receptor (GPCR) with high affinity for sphingosine 1-phosphate (S1P) . Although EDG1 has been reported to link to multiple signaling pathways , no role in TCR signaling had been documented. From our genetic screen, we obtained two carboxy-terminal truncation EDG1 mutants. Reintroducing EDG1 Hit 1 into naïve Jurkat cells conferred a CD69-inhibition phenotype (Figure 4d). We believe the EDG1 hits may work as constitutively active forms of the endogenous protein, given that overexpressing full-length EDG1 also caused inhibition of CD69 expression (data not shown).
PAK (p21-activated kinase) proteins are critical effectors that link Rho-family GTPases, such as Cdc42 and Rac1, to cytoskeletal reorganization and nuclear signaling [18, 19]. PAK proteins constitute a family of serine/threonine kinases that utilizes the CRIB (Cdc42/Rac interactive binding) domain to bind to small GTPases; members of the family include PAK1, PAK2, PAK3 and PAK4 . Among the four PAK proteins, PAK2 (also known as PAK65  and gamma-PAK ) is activated by proteolytic cleavage during caspase-mediated apoptosis . The role of PAK2 in Jurkat T cells has been reported primarily to be in membrane and morphological changes in apoptotic cells . PAK1, on the other hand, has been reported to be involved in T-cell signaling [24, 25]. Interestingly, we identified two different truncated versions of PAK2, both lacking the kinase domain, in our functional genetic screens with the fourth highest frequency (after TCRβ, ZAP70 and TCPTP; see Table 1). We further demonstrated that these dominant-negative forms of PAK2 also confer CD69 inhibition when introduced into naïve Jurkat cells (Figure 4e and Table 1).
An interesting adaptor molecule cloned from our genetic screen is Grb7 (Figure 4f). Like Grb2, Grb7 was originally cloned by screening bacterial expression libraries with the tyrosine-phosphorylated carboxyl terminus of the epidermal growth factor (EGF) receptor . The Grb7 family of proteins - Grb7, Grb10, and Grb14 - share significant sequence homology and a conserved molecular architecture . Their functional domains include a proline-rich region, an RA (RalGEF/AF6 or Ras-associating) domain, a PH domain and an SH2 domain. Like other adaptor molecules, Grb7 family proteins function to mediate the coupling of multiple cell-surface receptors to downstream signaling pathways in the regulation of various cellular functions. Our identification of a strong phenotype for the Grb7 SH2 domain in TCR signal transduction suggests that Grb7 may be an important immune-regulatory molecule (Figure 4f).
We also discovered an uncharacterized molecule whose sequence in GenBank was assembled from expressed sequence tag (EST) data. This novel molecule, FLJ20456, was renamed by us as TRAC-1, for T-cell RING protein in activation. As shown in Figure 4g, TRAC-1 has a RING finger domain, which is characteristically found in a class of proteins collectively called ubiquitin ligases or E3s . Members of the Cbl protein family are the best-known E3s involved in the regulation of TCR signaling . T cells manifest enhanced signaling in both c-Cbl and Cbl-b mutant mice, suggesting that the wild-type function of these proteins is in negatively regulating T-cell activation. More recently, Cbl proteins have been shown to function as RING finger E3s so as specifically to target activated receptors and protein-tyrosine kinases for ubiquitination and therefore to down-regulate their signaling . The TRAC-1 hit we obtained has a truncation in the carboxyl terminus but still retains the intact RING finger domain (Figure 4g). Reintroducing the TRAC-1 hit into naïve Jurkat cells caused strong inhibition of the anti-TCR-induced CD69 expression in infected cells.
For a complete characterization of the functional genetic screen, as well as additional selected hits, see Additional data files 6 and 7.
Gene expression in tissues and primary lymphocytes
We further examined expression of these selected genes in lymphocyte subsets isolated from healthy human peripheral blood using semi-quantitative RT-PCR. As shown in Figure 5c, EDG1 expression was detected in both T cells (higher expression in CD4+ than in CD8+ T cells) and B cells (CD19+), but not in monocytes (CD14+). Its expression level in T and B cells was not affected upon mitogenic activation. EDG1 was also detected in the brain. PAK2 was detected in resting and activated lymphocytes as well as in the placenta (Figure 5d). Even though Grb7 was not detected in the PBL by northern blot, it was detected in peripheral blood mononuclear cells (PBMC) using the more sensitive RT-PCR method (Figure 5e). Grb7 expression seemed to be slightly increased upon activation. Consistent with the northern blot profile, TRAC-1 was detected only in lymphocytes and not in the placenta (Figure 5f). In summary, all four genes are expressed in the lymphoid system, supporting their potential physiological role in lymphocyte signaling.
Function in primary T lymphocytes
As seen with Jurkat cells (data not shown), GFP translated by way of IRES was not as abundant as GFP translated using the conventional Kozak sequence (comparing GFP geometric mean from CRU5-IRES-GFP to that from CRU5-GFP). Nevertheless, the percentage infection remained similar (Figure 6b; 32.4% and 31.3% respectively). Insertion of a gene in front of IRES-GFP further reduced the expression level of GFP (Figure 6b), a trend observed with many other cell lines (data not shown). After allowing cells to rest for 5 days following infection, we flow-sorted cells into two populations: GFP-negative and GFP-positive. Exact numbers of sorted cells were immediately put into culture. As seen in Figure 6c, resting cells did not produce IL-2, nor did cells stimulated with anti-CD3 alone. Anti-CD3 plus anti-CD28 induced robust IL-2 production in the CIG vector-infected cells (CIG), regardless of the GFP expression (note the different scales of the upper graphs compared to the lower ones).
These observations are consistent with previous reports on freshly isolated primary T lymphocytes and also indicate that prior culture and retroviral infection did not change the basic properties of these primary T lymphocytes. Addition of anti-CD28 in conjunction with anti-CD3 also led to high IL-2 production from the GFP-negative population of cells infected with CIG-LCK, -PLCγ1, -EDG1 and -PAK2 hits. The GFP-positive population from these cells was, however, significantly impaired in IL-2 production following anti-CD3 and anti-CD28 stimulation (Figure 6c). As expected, the defect caused by the Lck, PLCγ1, EDG1 and PAK2 hits can be completely rescued by stimulation using PMA and ionomycin (Figure 6c). Taken together, these results show that Lck and PLCγ1 play a crucial role in IL-2 production from primary T lymphocytes, consistent with their involvement in membrane-proximal signaling events of T-cell activation. More importantly, our results document for the first time the involvement of the seven-transmembrane molecule EDG1 and the serine/threonine kinase PAK2 in physiological functions of T cells. Together, the results also demonstrate a rapid system for further validating hits from functional genetic screens using primary lymphocytes.
In this article, we report a large-scale functional genetic screen for inhibitors of TCR signaling. We isolated many known signaling molecules - such as Lck, ZAP70, Syk, PLCγ1 - as novel truncation mutants (probably created during library preparation) with dominant-negative effects. In addition, we also discovered molecules previously unknown to this pathway, including transmembrane molecules (EDG-1, IL-10Rα and integrin α2), cytoplasmic enzymes and adaptors (PAK-2, A-Raf-1, TCPTP, Grb7, SH2-B and GG2-1), and cytoskeletal molecules (moesin and vimentin; see Table 1). Of note, we also identified a novel molecule, TRAC-1, which had lymphoid and hematopoietic specific expression (Figure 5b).
We showed that EDG1, PAK2, and Grb7, genes originally described in different contexts, are also expressed in lymphocytes (Figure 5). This is not unexpected, since the retroviral cDNA libraries were generated using mRNA from human lymphoid organs such as thymus, spleen, lymph nodes and bone marrow. Our expression data are generally consistent with those published by other investigators. For example, EDG1 was reported to be expressed in human natural killer cells  and dendritic cells . PAK2 is expressed ubiquitously in human tissues  and in Jurkat cells . Grb7 has a broad expression in human (pancreas, placenta, kidney, prostate and small intestines) . Grb7 was not easily detectable by northern blot in thymus, spleen and PBL, but its expression was detected in specific lymphocyte subsets (Figure 5e). This indicates that our screen is capable of identifying genes with potentially important roles in lymphocyte activation whose expression is not limited to the lymphoid system. The fact that these genes' expression is not limited to the lymphoid system does not diminish the potential role they could play in lymphocyte activation. For example, the Ras-Raf-MAP kinase pathway is ubiquitously present in many tissues and cell types, as well as conserved evolutionarily, but this pathway has also been shown to be important in lymphocyte signaling.
In the 'post-genomics' era, the novelty of discovery lies in assigning novel functions to gene products. In our screens, for example, we identified two hits representing cytoplasmic truncated versions of EDG-1, a receptor for S1P . Interestingly, FTY720, a potent immunosuppressant in advanced clinical development, has been shown to act through EDG-1 and S1P signaling pathways [35, 36]. The fact that truncated EDG-1 proteins were identified in our T-cell activation screen suggests potential intersections of the TCR signaling pathway and the S1P signaling pathway, as well as new insights into the mechanisms of action of FTY720.
Our results also call for attention to potential differences between related family members. For example, PAK-1 (instead of PAK-2), c-Raf-1 (instead of A-Raf-1) and Grb2 (instead of Grb7) have been reported to be associated with the TCR signal transduction pathway [25, 37–40]. Our functional genetic screens identified PAK2, A-Raf-1, and Grb7 as important regulators of TCR-induced CD69 expression. It is possible that the dominant-negative proteins we cloned also inhibit other related family members. Alternatively, it is equally possible that the previously reported dominant-negative forms of PAK1, c-Raf-1, and to a lesser extent, Grb2, may have inhibited PAK2, A-Raf-1 and Grb7, respectively. In fact, binding of the human immunodeficiency virus (HIV) Nef protein and subsequent activation of the PAK-related kinase and phosphorylation of its substrate can be readily detected in both infected primary T lymphocytes and macrophages . When the HIV-Nef-associated kinase was characterized carefully, it became clear that this kinase was PAK2 and not PAK1 [42, 43]. This example supports the notion that PAK2 could be the more relevant kinase in T-cell signaling. Of course, it is entirely possible that these related family members are not mutually exclusive in participating in the TCR signal-transduction pathway.
In conclusion, we have demonstrated a successful approach for discovering and validating, in a functionally relevant context, important immune regulators on a genome-wide scale. This approach provides a tool for functional cloning of regulators in numerous signal-transduction pathways [44, 45]. For example, B-cell activation-induced CD69 expression  and, recently, the IL-4-induced immunoglobulin E class switch , have also been shown to be amenable to genetic perturbation following introduction of retroviral cDNA or random cyclic peptide libraries. Importantly, the outlined strategy, which requires no prior sequence information of the players involved, does not bias the search to previously known signaling molecules, molecules flagged by DNA-array technologies, or signaling molecules discovered in other contexts. This approach has added to the list of potential players in T-cell biology that have not been identified in other standard pathway-mapping techniques.
Materials and methods
Preparation of cDNA libraries
The mRNA extracted from human lymph nodes, thymus, spleen and bone marrow was used to produce two randomly primed cDNA libraries. For one library (-ATG) inserts were directionally cloned and the second (+ATG) non-directionally cloned and provided with three exogenous ATGs in three frames. The resulting cDNAs were cloned into the pTRA-exs vector  for doxycycline-(Dox-) regulatable expression in cell lines expressing the tetracycline transactivator protein (tTA) . The total combined complexity of the two pTRA-cDNA libraries was 5 × 107 independent clones.
Phoenix A cells were cultured in DMEM supplemented with 10% fetal calf serum (FCS), penicillin and streptomycin. Human T-cell leukemia line Jurkat was obtained from Novartis (Vienna, Austria) and was cultured in RPMI 1640 medium supplemented with 10% FCS, penicillin and streptomycin. Clone 4D9 with an optimal CD69 induction was obtained after sequential FACS-sorting for low basal CD69 expression and high induction of CD69 expression following TCR stimulation. To produce the Jurkat-tTA cell line, Clone 4D9 was infected with a reporter construct which expresses Lyt2 driven by a tetracycline responsive element (TRE) and a retroviral construct, CtTA1H, which constitutively expresses tTA . The Jurkat-tTA cell clone 4D9#32 was obtained by sorting for high Lyt2 expression in the absence of Dox and low expression of Lyt2 in the presence Dox (10 ng/ml).
Transfection and infection
Phoenix A packaging cells were transfected with retroviral vectors using calcium phosphate for 6 h following standard protocols . After 24 h, supernatant was replaced with complete RPMI medium and virus was allowed to accumulate for 24 h at 32°C. Viral supernatant was collected, filtered through a 0.2 μM filter and mixed with Jurkat cells or human primary T lymphocytes at a density of 5 × 105 cells per ml. Cells were spun at room temperature for 3 h at 2,500 rpm, followed by overnight incubation at 37°C. Transfection and infection efficiencies were monitored by FACS. Functional analysis was carried out at least 2 days after infection.
For CD69 upregulation experiments, Jurkat cells were split to 2.5 × 105 cells per ml 24 h prior to stimulation. Cells were spun and resuspended at 5 × 105 cells per ml in fresh complete RPMI medium in the presence of 300 ng/ml C305 (anti-Jurkat clonotypic TCR) hybridoma  supernatant, 100 ng/ml OKT3 (anti-CD3), 100 ng/ml SpvT3 (anti-CD3), or PMA (5 ng/ml) for 20-26 h at 37°C, and then assayed for surface CD69 expression.
Antibodies and flow cytometry
Jurkat cells or human peripheral blood lymphocytes were stained with FITC-conjugated monoclonal anti-mouse CD8α (Lyt2), APC-conjugated mouse monoclonal anti-human CD3, anti-human CD8, or anti-human CD69 antibodies, and PE-conjugated mouse monoclonal anti-human CD3 or anti-CD4 antibodies (all from Caltag, Burlingame, USA) at 4°C for 20 min and analyzed using a FACSCalibur instrument (Becton Dickinson, Franklin Lakes, USA) with the CellQuest software. Fluorescent-activated cell sortings were performed on the MoFlo instruments (Cytomation, Fort Collins, USA).
Phoenix A packaging cells were transfected with pTRA-cDNA libraries. Supernatant containing packaged viral particles was used to infect 3.5 × 108 Jurkat-tTA cells with an efficiency of 52% based on parallel infection with TRA-GFP . After 4 days of cDNA expression, library-infected cells were stimulated with 300 ng/ml C305 for 20-30 h, stained with APC-conjugated anti-CD69 and PE-conjugated anti-CD3, and 1% of total cells with the desired CD69lowCD3+ phenotype were isolated using MoFlo. Sorting was repeated multiple times with a 6-day rest period between stimulations until the population was significantly enriched for the desired CD69lowCD3+ phenotype. Single cells were deposited to 96-well plates and expanded in the presence and absence of Dox, stimulated and analyzed for CD69 upregulation.
Isolation of cDNA inserts
PCR primers were designed to specifically amplify the inserts from pTRA-cDNA libraries. The primers contained flanking BstXI sites for subsequent cloning to the pTRA-IRES-GFP and CRU5-IRES-GFP vectors [48, 50]. BstXTRA5G: 5'-TTGCAGAACCACCACCTTGGGCTCTTAACCTAGGCCGATC-3'. BstXTRA3D: 5'-TTGCAGAACCAATTTAATGGCGGCCAGTCAGGCCATCGTCG-3'. RT-PCR cloning was achieved with kits from Clontech (Palo Alto, USA) or Life Technologies (Carlsbad, USA). The gel-purified RT-PCR fragments were sequenced as well as digested with BstXI for subcloning into the retroviral pTRA-IRES-GFP or CRU5-IRES-GFP vectors.
Semi-quantitative PCR analysis
Human Blood Fractions MTC panel (Clontech) with normalized, first-strand cDNA preparations from RNA of various purified cells were used as templates. CD19+ cells were activated with 2 μl/ml pokeweed mitogen for 4 days, mononuclear cells with 2 μl/ml pokeweed mitogen and 5 μg/ml concanavalin A for 3 days, CD4+ cells with 5 μg/ml concanavalin A for 3-4 days, and CD8+ cells with 5 μg/ml phytohemagglutinin for 3 days. The following primers were used to amplify various cDNA fragments: EDG1: forward primer 5'-GCAAGAACATTTCCAAGGCCAGCC-3', reverse primer 5'-GGGTGTGGGATGTACAGGGCATCC-3', 35 cycles; PAK2: forward primer 5'-CGGAGAACTGGAAGATAAGCCTCC-3', reverse primer 5'-AAAGCCAACATGGATGGTGTGCTC-3', 35 cycles; Grb7: forward primer 5'-ATGCCCACTGACTTCGGTTT-3', reverse primer 5'-GATCCGAAGCCCCTTGTGT-3', 40 cycles; TRAC-1: forward primer 5'-TTACACCAGCCTGTCCGGA-3', reverse primer 5'-CAGACTGGTAGCAATACAGGAACG-3', 35 cycles.
Commercially available primers were used for GAPDH (PerkinElmer, Wellesley, USA) and β-actin (Clontech), 25 cycles. The PCR products were then electrophoresed on agarose/ethidium bromide gels.
Northern blot analysis
Human Multiple Tissue Northern Blots were purchased from Clontech. The following probes were used: EDG1, base pairs 1-1,023 of its open reading frame; PAK2, base pairs 1-341 of its open reading frame; Grb7, base pairs 1,268-1,599 of its open reading frame; and TRAC-1, base pairs 1-509 of its open reading frame.
Culture and infection of primary T lymphocytes
Commercially available primary blood mononuclear cells (PBMC; AllCells LLC, Berkeley, USA) were cultured in RPMI + 10% FCS for 1-2 h in tissue culture flasks to allow macrophages and other adhering cells to settle down. The suspended cells were cultured with anti-CD3 (30 ng/ml) and anti-CD28 (100 ng/ml) for 2 days to allow T cells to expand and other cell types to gradually die off. These primary T lymphocytes were infected with 1 ml retroviral supernatant in a 24-well plate. One day after infection, 1 ml spent medium from the bulk culture was added to each well. The cells were further expanded for a few days with addition of fresh RPMI + 10% FCS. Such an expansion also allowed the cells to return to the resting state with low CD69, CD25, and CD40L expression. Cells were then sorted by FACS, on the basis of GFP expression, directly into a round bottom 96-well plate coated with anti-CD3 alone, anti-CD3 + anti-CD28, or not coated. To the uncoated wells, PMA (5 ng/ml final) and ionomycin (1 μM final) were added. Then, 40 h later, supernatants were harvested for IL-2 measurement using commercial reagents (R&D Systems, Minneapolis, USA).
Additional data files
The following are provided as additional materials: details of the selection and infection of Jurkat clone 4D9 (Additional data file 1); construction of the pTRA-cDNA libraries and assessing the efficiency of infection (Additional data file 2); distinction between CD3-, CD3low and CD3high cell populations (Additional data file 3); distribution of Dox ratios among the 2,828 single-cell clones analyzed (Additional data file 4); details of clones with TCRβ hits (Additional data file 5); a summary of the genetic screen for inhibitors of TCR-induced CD69 expression (Additional data file 6); characterization of additional hits from the T-cell activation screen (Additional data file 7); correlation of the CD69 inhibitory phenotype with the cDNA expression level (Additional data file 8).
We thank Arthur Weiss (UCSF) for providing the C305 hybridoma and ZAP70 constructs used for positive controls, our collaborators Ulf Korthaeuer, Max Woisetschlager, Christoph Heusser, Jutta Heim and N. Rao Movva from Novartis for their support during this work. We thank Jim Lorens, Anup Nagin, Sacha Holland, Xian Wu, Monette Aujay, and Mel Fox for their excellent technical advice and assistance, Jianing Huang and Garry Nolan for critical reading of the manuscript, and Louis Tamayo, Carine Richards, and Amelia Cervantes for assistance with the preparation of the manuscript.
- Marrack P, Kappler J: The T cell receptor. Science. 1987, 238: 1073-1079.PubMedView Article
- Weiss A, Stobo JD: Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J Exp Med. 1984, 160: 1284-1299.PubMedView Article
- Dustin ML, Chan AC: Signaling takes shape in the immune system. Cell. 2000, 103: 283-294.PubMedView Article
- Kane LP, Lin J, Weiss A: Signal transduction by the TCR for antigen. Curr Opin Immunol. 2000, 12: 242-249. 10.1016/S0952-7915(00)00083-2.PubMedView Article
- Singer AL, Koretzky GA: Control of T cell function by positive and negative regulators. Science. 2002, 296: 1639-1640. 10.1126/science.1071551.PubMedView Article
- Ziegler SF, Ramsdell F, Alderson MR: The activation antigen CD69. Stem Cells. 1994, 12: 456-465.PubMedView Article
- Yablonski D, Kuhne MR, Kadlecek T, Weiss A: Uncoupling of nonreceptor tyrosine kinases from PLC-gamma1 in an SLP-76-deficient T cell. Science. 1998, 281: 413-416. 10.1126/science.281.5375.413.PubMedView Article
- Zhang W, Irvin BJ, Trible RP, Abraham RT, Samelson LE: Functional analysis of LAT in TCR-mediated signaling pathways using a LAT-deficient Jurkat cell line. Int Immunol. 1999, 11: 943-950. 10.1093/intimm/11.6.943.PubMedView Article
- Baron U, Gossen M, Bujard H: Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. Nucleic Acids Res. 1997, 25: 2723-2729. 10.1093/nar/25.14.2723.PubMedPubMed CentralView Article
- Qian D, Mollenauer MN, Weiss A: Dominant-negative zeta-associated protein 70 inhibits T cell antigen receptor signaling. J Exp Med. 1996, 183: 611-620.PubMedView Article
- Lin J, Weiss A: T cell receptor signalling. J Cell Sci. 2001, 114: 243-244.PubMed
- Straus DB, Weiss A: Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell. 1992, 70: 585-593.PubMedView Article
- Secrist JP, Karnitz L, Abraham RT: T-cell antigen receptor ligation induces tyrosine phosphorylation of phospholipase C-gamma 1. J Biol Chem. 1991, 266: 12135-12139.PubMed
- Irvin BJ, Williams BL, Nilson AE, Maynor HO, Abraham RT: Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling: reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol Cell Biol. 2000, 20: 9149-9161. 10.1128/MCB.20.24.9149-9161.2000.PubMedPubMed CentralView Article
- Hla T, Maciag T: An abundant transcript induced in differentiating human endothelial cells encodes a polypeptide with structural similarities to G-protein-coupled receptors. J Biol Chem. 1990, 265: 9308-9313.PubMed
- Lee MJ, Van Brocklyn JR, Thangada S, Liu CH, Hand AR, Menzeleev R, Spiegel S, Hla T: Sphingosine-1-phosphate as a ligand for the G protein-coupled receptor EDG-1. Science. 1998, 279: 1552-1555. 10.1126/science.279.5356.1552.PubMedView Article
- Okamoto H, Takuwa N, Gonda K, Okazaki H, Chang K, Yatomi Y, Shigematsu H, Takuwa Y: EDG1 is a functional sphingosine-1-phosphate receptor that is linked via a Gi/o to multiple signaling pathways, including phospholipase C activation, Ca2+ mobilization, Ras-mitogen-activated protein kinase activation, and adenylate cyclase inhibition. J Biol Chem. 1998, 273: 27104-27110. 10.1074/jbc.273.42.27104.PubMedView Article
- Lim L, Manser E, Leung T, Hall C: Regulation of phosphorylation pathways by p21 GTPases. The p21 Ras-related Rho subfamily and its role in phosphorylation signalling pathways. Eur J Biochem. 1996, 242: 171-185.PubMedView Article
- Bokoch GM: Biology of the p21-activated kinases. Annu Rev Biochem. 2003, 72: 743-781. 10.1146/annurev.biochem.72.121801.161742.PubMedView Article
- Martin GA, Bollag G, McCormick F, Abo A: A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20. EMBO J. 1995, 14: 1970-1978.PubMedPubMed Central
- Teo M, Manser E, Lim L: Identification and molecular cloning of a p21cdc42/rac1-activated serine/threonine kinase that is rapidly activated by thrombin in platelets. J Biol Chem. 1995, 270: 26690-26697. 10.1074/jbc.270.44.26690.PubMedView Article
- Rudel T, Bokoch GM: Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2. Science. 1997, 276: 1571-1574. 10.1126/science.276.5318.1571.PubMedView Article
- Rudel T, Zenke FT, Chuang TH, Bokoch GM: p21-activated kinase (PAK) is required for Fas-induced JNK activation in Jurkat cells. J Immunol. 1998, 160: 7-11.PubMed
- Bubeck Wardenburg J, Pappu R, Bu JY, Mayer B, Chernoff J, Straus D, Chan AC: Regulation of PAK activation and the T cell cytoskeleton by the linker protein SLP-76. Immunity. 1998, 9: 607-616.PubMedView Article
- Yablonski D, Kane LP, Qian D, Weiss A: A Nck-Pak1 signaling module is required for T-cell receptor-mediated activation of NFAT, but not of JNK. EMBO J. 1998, 17: 5647-5657. 10.1093/emboj/17.19.5647.PubMedPubMed CentralView Article
- Margolis B, Silvennoinen O, Comoglio F, Roonprapunt C, Skolnik E, Ullrich A, Schlessinger J: High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains. Proc Natl Acad Sci USA. 1992, 89: 8894-8898.PubMedPubMed CentralView Article
- Han DC, Shen TL, Guan JL: The Grb7 family proteins: structure, interactions with other signaling molecules and potential cellular functions. Oncogene. 2001, 20: 6315-6321. 10.1038/sj.onc.1204775.PubMedView Article
- Joazeiro CA, Weissman AM: RING finger proteins: mediators of ubiquitin ligase activity. Cell. 2000, 102: 549-552.PubMedView Article
- Ben-Neriah Y: Regulatory functions of ubiquitination in the immune system. Nat Immunol. 2002, 3: 20-26. 10.1038/ni0102-20.PubMedView Article
- Sawasdikosol S, Pratt JC, Meng W, Eck MJ, Burakoff SJ: Adapting to multiple personalities: Cbl is also a RING finger ubiquitin ligase. Biochim Biophys Acta. 2000, 1471: M1-M12. 10.1016/S0304-419X(00)00013-5.PubMed
- Demo SD, Masuda E, Rossi AB, Throndset BT, Gerard AL, Chan EH, Armstrong RJ, Fox BP, Lorens JB, Payan DG, et al: Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assay. Cytometry. 1999, 36: 340-348. 10.1002/(SICI)1097-0320(19990801)36:4<340::AID-CYTO9>3.0.CO;2-C.PubMedView Article
- Kveberg L, Bryceson Y, Inngjerdingen M, Rolstad B, Maghazachi AA: Sphingosine 1 phosphate induces the chemotaxis of human natural killer cells. Role for heterotrimeric G proteins and phosphoinositide 3 kinases. Eur J Immunol. 2002, 32: 1856-1864. 10.1002/1521-4141(200207)32:7<1856::AID-IMMU1856>3.0.CO;2-B.PubMedView Article
- Idzko M, Panther E, Corinti S, Morelli A, Ferrari D, Herouy Y, Dichmann S, Mockenhaupt M, Gebicke-Haerter P, Di Virgilio F, et al: Sphingosine 1-phosphate induces chemotaxis of immature and modulates cytokine-release in mature human dendritic cells for emergence of Th2 immune responses. FASEB J. 2002, 16: 625-627.PubMed
- Frantz JD, Giorgetti-Peraldi S, Ottinger EA, Shoelson SE: Human GRB-IRbeta/GRB10. Splice variants of an insulin and growth factor receptor-binding protein with PH and SH2 domains. J Biol Chem. 1997, 272: 2659-2667. 10.1074/jbc.272.2.894.PubMedView Article
- Brinkmann V, Davis MD, Heise CE, Albert R, Cottens S, Hof R, Bruns C, Prieschl E, Baumruker T, Hiestand P, et al: The immune modulator FTY720 targets sphingosine 1-phosphate receptors. J Biol Chem. 2002, 277: 21453-21457. 10.1074/jbc.C200176200.PubMedView Article
- Mandala S, Hajdu R, Bergstrom J, Quackenbush E, Xie J, Milligan J, Thornton R, Shei GJ, Card D, Keohane C, et al: Alteration of lymphocyte trafficking by sphingosine-1-phosphate receptor agonists. Science. 2002, 296: 346-349. 10.1126/science.1070238.PubMedView Article
- Izquierdo M, Bowden S, Cantrell D: The role of Raf-1 in the regulation of extracellular signal-regulated kinase 2 by the T cell antigen receptor. J Exp Med. 1994, 180: 401-406.PubMedView Article
- Siegel JN, June CH, Yamada H, Rapp UR, Samelson LE: Rapid activation of C-Raf-1 after stimulation of the T-cell receptor or the muscarinic receptor type 1 in resting T cells. J Immunol. 1993, 151: 4116-4127.PubMed
- Reif K, Buday L, Downward J, Cantrell DA: SH3 domains of the adapter molecule Grb2 complex with two proteins in T cells: the guanine nucleotide exchange protein Sos and a 75-kDa protein that is a substrate for T cell antigen receptor-activated tyrosine kinases. J Biol Chem. 1994, 269: 14081-14087.PubMed
- Buday L, Egan SE, Rodriguez Viciana P, Cantrell DA, Downward J: A complex of Grb2 adaptor protein, Sos exchange factor, and a 36-kDa membrane-bound tyrosine phosphoprotein is implicated in ras activation in T cells. J Biol Chem. 1994, 269: 9019-9023.PubMed
- Brown A, Wang X, Sawai E, Cheng-Mayer C: Activation of the PAK-related kinase by human immunodeficiency virus type 1 Nef in primary human peripheral blood lymphocytes and macrophages leads to phosphorylation of a PIX-p95 complex. J Virol. 1999, 73: 9899-9907.PubMedPubMed Central
- Renkema GH, Manninen A, Mann DA, Harris M, Saksela K: Identification of the Nef-associated kinase as p21-activated kinase 2. Curr Biol. 1999, 9: 1407-1410. 10.1016/S0960-9822(00)80086-X.PubMedView Article
- Arora VK, Molina RP, Foster JL, Blakemore JL, Chernoff J, Fredericksen BL, Garcia JV: Lentivirus Nef specifically activates Pak2. J Virol. 2000, 74: 11081-11087. 10.1128/JVI.74.23.11081-11087.2000.PubMedPubMed CentralView Article
- Lorens JB, Sousa C, Bennett MK, Molineaux SM, Payan DG: The use of retroviruses as pharmaceutical tools for target discovery and validation in the field of functional genomics. Curr Opin Biotechnol. 2001, 12: 613-621. 10.1016/S0958-1669(01)00269-5.PubMedView Article
- Xu X, Leo C, Jang Y, Chan E, Padilla D, Huang BC, Lin T, Gururaja T, Hitoshi Y, Lorens JB, et al: Dominant effector genetics in mammalian cells. Nat Genet. 2001, 27: 23-29. 10.1038/83717.PubMedView Article
- Holland SJ, Liao XC, Mendenhall MK, Zhou X, Pardo J, Chu P, Spencer C, Fu A, Sheng N, Yu P, et al: Functional cloning of Src-like adapter protein-2 (SLAP-2), a novel inhibitor of antigen receptor signaling. J Exp Med. 2001, 194: 1263-1276. 10.1084/jem.194.9.1263.PubMedPubMed CentralView Article
- Kinsella TM, Ohashi CT, Harder AG, Yam GC, Li W, Peelle B, Pali ES, Bennett MK, Molineaux SM, Anderson DA, et al: Retrovirally delivered random cyclic peptide libraries yield inhibitors of interleukin-4 signaling in human B cells. J Biol Chem. 2002, 277: 37512-37518. 10.1074/jbc.M206162200.PubMedView Article
- Lorens JB, Bennett MK, Pearsall DM, Throndset WR, Rossi AB, Armstrong RJ, Fox BP, Chan EH, Luo Y, Masuda E, et al: Retroviral delivery of peptide modulators of cellular functions. Mol Ther. 2000, 1: 438-447. 10.1006/mthe.2000.0063.PubMedView Article
- Swift SE, Lorens JB, Achacoso P, Nolan GP: Rapid production of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems. In Current Protocols in Immunology. Edited by: Coligan RCJE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W. 2000, Hoboken, NJ: John Wiley and Sons, 10.17C: 1-17.
- Hitoshi Y, Lorens J, Kitada SI, Fisher J, LaBarge M, Ring HZ, Francke U, Reed JC, Kinoshita S, Nolan GP: Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells. Immunity. 1998, 8: 461-471.PubMedView Article
- GenBank. [http://www.ncbi.nlm.nih.gov/Genbank/]
- Liu H, Rhodes M, Wiest DL, Vignali DA: On the dynamics of TCR:CD3 complex cell surface expression and downmodulation. Immunity. 2000, 13: 665-675.PubMedView Article
- Law CL, Sidorenko SP, Chandran KA, Draves KE, Chan AC, Weiss A, Edelhoff S, Disteche CM, Clark EA: Molecular cloning of human Syk. A B cell protein-tyrosine kinase associated with the surface immunoglobulin M-B cell receptor complex. J Biol Chem. 1994, 269: 12310-12319.PubMed
- Williams BL, Schreiber KL, Zhang W, Wange RL, Samelson LE, Leibson PJ, Abraham RT: Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T-cell line. Mol Cell Biol. 1998, 18: 1388-1399.PubMedPubMed CentralView Article
- Fargnoli J, Burkhardt AL, Laverty M, Kut SA, van Oers NS, Weiss A, Bolen JB: Syk mutation in Jurkat E6-derived clones results in lack of p72syk expression. J Biol Chem. 1995, 270: 26533-26537. 10.1074/jbc.270.44.26533.PubMedView Article
- Shultz LD, Rajan TV, Greiner DL: Severe defects in immunity and hematopoiesis caused by SHP-1 protein-tyrosine-phosphatase deficiency. Trends Biotechnol. 1997, 15: 302-307. 10.1016/S0167-7799(97)01060-3.PubMedView Article
- Ravetch JV, Lanier LL: Immune inhibitory receptors. Science. 2000, 290: 84-89. 10.1126/science.290.5489.84.PubMedView Article
- Yang J, Cheng Z, Niu T, Liang X, Zhao ZJ, Zhou GW: Structural basis for substrate specificity of protein-tyrosine phosphatase SHP-1. J Biol Chem. 2000, 275: 4066-4071. 10.1074/jbc.275.6.4066.PubMedView Article
- Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, et al: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000, 191: 1591-1604. 10.1084/jem.191.9.1591.PubMedPubMed CentralView Article
- Kawabuchi M, Satomi Y, Takao T, Shimonishi Y, Nada S, Nagai K, Tarakhovsky A, Okada M: Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases. Nature. 2000, 404: 999-1003. 10.1038/35010121.PubMedView Article
- Zhang W, Sloan-Lancaster J, Kitchen J, Trible RP, Samelson LE: LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation. Cell. 1998, 92: 83-92.PubMedView Article
- Torgersen KM, Vang T, Abrahamsen H, Yaqub S, Horejsi V, Schraven B, Rolstad B, Mustelin T, Tasken K: Release from tonic inhibition of T cell activation through transient displacement of C-terminal Src kinase (Csk) from lipid rafts. J Biol Chem. 2001, 276: 29313-29318. 10.1074/jbc.C100014200.PubMedView Article
- Hermiston ML, Xu Z, Majeti R, Weiss A: Reciprocal regulation of lymphocyte activation by tyrosine kinases and phosphatases. J Clin Invest. 2002, 109: 9-14. 10.1172/JCI200214794.PubMedPubMed CentralView Article
- Itoh K, Sakakibara M, Yamasaki S, Takeuchi A, Arase H, Miyazaki M, Nakajima N, Okada M, Saito T: Cutting edge: negative regulation of immune synapse formation by anchoring lipid raft to cytoskeleton through Cbp-EBP50-ERM assembly. J Immunol. 2002, 168: 541-544.PubMedView Article
- Kolch W: Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions. Biochem J. 2000, 351: 289-305. 10.1042/0264-6021:3510289.PubMedPubMed CentralView Article
- Izquierdo M, Bowden S, Cantrell D: The role of Raf-1 in the regulation of extracellular signal-regulated kinase 2 by the T cell antigen receptor. J Exp Med. 1994, 180: 401-406.PubMedView Article
- Gupta S, Weiss A, Kumar G, Wang S, Nel A: The T-cell antigen receptor utilizes Lck, Raf-1, and MEK-1 for activating mitogen-activated protein kinase. Evidence for the existence of a second protein kinase C-dependent pathway in an Lck-negative Jurkat cell mutant. J Biol Chem. 1994, 269: 17349-17357.PubMed
- Kolch W, Heidecker G, Lloyd P, Rapp UR: Raf-1 protein kinase is required for growth of induced NIH/3T3 cells. Nature. 1991, 349: 426-428. 10.1038/349426a0.PubMedView Article
- Schaap D, van der Wal J, Howe LR, Marshall CJ, van Blitterswijk WJ: A dominant-negative mutant of raf blocks mitogen-activated protein kinase activation by growth factors and oncogenic p21ras. J Biol Chem. 1993, 268: 20232-20236.PubMed
- Cool DE, Tonks NK, Charbonneau H, Walsh KA, Fischer EH, Krebs EG: cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family. Proc Natl Acad Sci USA. 1989, 86: 5257-5261.PubMedPubMed CentralView Article
- Hao L, Tiganis T, Tonks NK, Charbonneau H: The noncatalytic C-terminal segment of the T cell protein tyrosine phosphatase regulates activity via an intramolecular mechanism. J Biol Chem. 1997, 272: 29322-29329. 10.1074/jbc.272.46.29322.PubMedView Article
- Liu Y, Wei SH, Ho AS, de Waal Malefyt R, Moore KW: Expression cloning and characterization of a human IL-10 receptor. J Immunol. 1994, 152: 1821-1829.PubMed
- Takada Y, Hemler ME: The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain. J Cell Biol. 1989, 109: 397-407.PubMedView Article
- Kolanus W, Seed B: Integrins and inside-out signal transduction: converging signals from PKC and PIP3. Curr Opin Cell Biol. 1997, 9: 725-731. 10.1016/S0955-0674(97)80127-5.PubMedView Article
- Obergfell A, Judd BA, del Pozo MA, Schwartz MA, Koretzky GA, Shattil SJ: The molecular adapter SLP-76 relays signals from platelet integrin alphaIIbbeta3 to the actin cytoskeleton. J Biol Chem. 2001, 276: 5916-5923. 10.1074/jbc.M010639200.PubMedView Article
- Peterson EJ, Woods ML, Dmowski SA, Derimanov G, Jordan MS, Wu JN, Myung PS, Liu QH, Pribila JT, Freedman BD, et al: Coupling of the TCR to integrin activation by Slap-130/Fyb. Science. 2001, 293: 2263-2265. 10.1126/science.1063486.PubMedView Article
- Siliciano JD, Morrow TA, Desiderio SV: itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2. Proc Natl Acad Sci USA. 1992, 89: 11194-11198.PubMedPubMed CentralView Article
- Liao XC, Littman DR: Altered T cell receptor signaling and disrupted T cell development in mice lacking Itk. Immunity. 1995, 3: 757-769.PubMedView Article
- Woods ML, Kivens WJ, Adelsman MA, Qiu Y, August A, Shimizu Y: A novel function for the Tec family tyrosine kinase Itk in activation of beta 1 integrins by the T-cell receptor. EMBO J. 2001, 20: 1232-1244. 10.1093/emboj/20.6.1232.PubMedPubMed CentralView Article
- Ivaska J, Reunanen H, Westermarck J, Koivisto L, Kahari VM, Heino J: Integrin alpha2beta1 mediates isoform-specific activation of p38 and upregulation of collagen gene transcription by a mechanism involving the alpha2 cytoplasmic tail. J Cell Biol. 1999, 147: 401-416. 10.1083/jcb.147.2.401.PubMedPubMed CentralView Article
- Osborne MA, Dalton S, Kochan JP: The yeast tribrid system - genetic detection of trans-phosphorylated ITAM-SH2-interactions. Biotechnology. 1995, 13: 1474-1478.PubMedView Article
- Huang X, Li Y, Tanaka K, Moore KG, Hayashi JI: Cloning and characterization of Lnk, a signal transduction protein that links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, and phosphatidylinositol 3-kinase. Proc Natl Acad Sci USA. 1995, 92: 11618-11622.PubMedPubMed CentralView Article
- Yokouchi M, Suzuki R, Masuhara M, Komiya S, Inoue A, Yoshimura A: Cloning and characterization of APS, an adaptor molecule containing PH and SH2 domains that is tyrosine phosphorylated upon B-cell receptor stimulation. Oncogene. 1997, 15: 7-15. 10.1038/sj.onc.1201163.PubMedView Article
- Takaki S, Sauer K, Iritani BM, Chien S, Ebihara Y, Tsuji K, Takatsu K, Perlmutter RM: Control of B cell production by the adaptor protein lnk. Definition of a conserved family of signal-modulating proteins. Immunity. 2000, 13: 599-609.PubMedView Article
- Horrevoets AJ, Fontijn RD, van Zonneveld AJ, de Vries CJ, ten Cate JW, Pannekoek H: Vascular endothelial genes that are responsive to tumor necrosis factor-alpha in vitro are expressed in atherosclerotic lesions, including inhibitor of apoptosis protein-1, stannin, and two novel genes. Blood. 1999, 93: 3418-3431.PubMed
- Kumar D, Whiteside TL, Kasid U: Identification of a novel tumor necrosis factor-alpha-inducible gene, SCC-S2, containing the consensus sequence of a death effector domain of fas-associated death domain-like interleukin-1beta-converting enzyme-inhibitory protein. J Biol Chem. 2000, 275: 2973-2978. 10.1074/jbc.275.4.2973.PubMedView Article
This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.