Dishevelled nuclear shuttling
© BioMed Central Ltd 2005
Published: 16 February 2005
Structure-function analysis of the Dishevelled (Dsh) protein in frog embryos has defined sequences that regulate Dsh nuclear localization, which proves critical for Wnt signaling.
Canonical and non-canonical Wnt pathways
During growth, development and disease, extracellular signals are communicated, or transduced, into the cell and in such a way as to elicit a particular cellular response. Many key signal transduction pathways have been dissected using genetic and biochemical approaches; such studies have defined the molecules that ensure signals initiated at the cell surface are efficiently transmitted to the cell nucleus, where they often result in the induction of a specific gene-expression program. Many signal transduction pathways are composed of modules that are remarkably conserved across species, such that lessons from different experimental model organisms have contributed to the understanding of molecular hierarchies that control signal communication in many cellular contexts.
Studies of the Wnt pathway provide a wonderful example of how researchers from different fields have contributed to a detailed understanding of a key signal transduction pathway . The Wnt pathway is critical for development and homeostasis of animals from Hydra to human ; Wnt signaling regulates cell proliferation, cell polarity and cell-fate determination. The Wnt signaling machinery is tightly regulated, and disruption of components of the signaling pathway have been implicated in diseases including cancer [2, 4].
The Dishevelled protein was first discovered in flies, and several homologs have been found in other organisms including mammals . Analysis of Dsh sequence alignments revealed the presence of three conserved domains called DIX, PDZ and DEP [5, 6], which are implicated in protein-protein interactions and targeting to subcellular sites. The modular design of the Dsh protein suggested that different domains might function to route Wnt-Frizzled signals in different directions.
"Once we had seen nuclear export we wanted to find out if there was an import signal," recalls Sokol. His team hunted for a conventional nuclear localization signal (NLS). "This was probably the most difficult part of the study, because the Dsh NLS doesn't match the known consensus." The team began a mutagenesis program, hacking away at the protein until they narrowed the NLS down to a short stretch of residues between the PDZ and DEP domains. "The Dsh NLS is atypical; it doesn't look like anything else," comments Sokol. "But it's very conserved between Dsh proteins across species, so it may be a specialized way for Dsh to get into the nucleus." Removing the NLS blocked nuclear import. NLS-mutant proteins also failed to induce secondary axes in frog embryos, or to stabilize β-catenin and activate downstream target genes. When the Dsh NLS was replaced with an unrelated NLS from a viral protein, Dsh activity was restored, as was canonical Wnt signaling. In contrast, the Dsh NLS mutation did not affect non-canonical Wnt signaling. Finally, Sokol's group showed that endogenous Dsh relocates to the nucleus in mammalian cells upon Wnt stimulation.
Dishevelled's nuclear shuffle
Sokol notes that some early reports mentioned nuclear localization of Dsh [7, 8], but these did not address the functional importance of Dsh nuclear accumulation in Wnt signaling. Randall Moon's group at the University of Washington in Seattle had noticed Dsh in the nucleus in association with the Dapper protein . "What is interesting is the Sokol finding that blocking nuclear export leads to accumulation in the nucleus, suggesting that Dsh nuclear accumulation is regulated," says Moon. "This is a careful study which provides compelling evidence for Dsh nuclear import," adds Howard Hughes Investigator Norbert Perrimon, who (independent from Sokol) works at Harvard Medical School.
"These results bring a new level of complexity to the regulation of the Wnt signaling pathway," agrees Patricia Salinas from University College London, UK. Her group previously showed that Dsh binds to microtubules and locally regulates signaling events in neuronal axons . She notes that a large number of Wnt signaling components have recently been found in the nucleus. "These results fit very well with our view that Dsh regulates distinct signaling events in specific cellular compartments. Our task now is to elucidate how the localization of Dishevelled is regulated. For example, what determines its re-localization to the nucleus or to microtubules?" Perrimon notes that "the issue of Dsh nuclear localization needs to be reexamined in the other systems to find out how general this is."
Sokol's results have met with some resistance from the Wnt community. Moon notes that often it takes years to change peoples' ideas about Wnt signaling. He cites the example of Frizzled receptors signaling via heterotrimeric G proteins, which he proposed years ago and which has only recently been clearly demonstrated. Sokol points out that β-catenin itself was originally described in cell adhesion. "People didn't believe that it goes to the nucleus until much later." Sokol is sure that many of the Wnt signaling proteins have nuclear functions.
"What remains completely opaque is what Dsh is doing in the nucleus and with whom," says Moon. Dsh has been reported to interact with over a dozen proteins, including several kinases . Sokol speculates that Dsh may have nuclear roles beyond the stabilization of β-catenin. For example, he notes that the recently identified Frodo protein binds to Dsh and Tcf proteins independent of β-catenin and may serve as a bridge to regulate gene expression . "I would say we are just at the tip of the iceberg," says Sokol. "There may be huge Dsh nuclear complexes that control chromatin structure or assembly." All in the field appear to agree that cellular localization offers possibilities for distinct functions in different compartments. "It remains a question whether Dsh mobilization to the nucleus depends on the cellular context or the amount of Wnt that the cell encounters," adds Salinas. "We truly understand only a fraction of the molecules involved in Wnt signaling," admits Moon. New technologies are revealing many new components of the Wnt β-catenin pathway. Several of these are nuclear and could interact with Dsh. "Thus many surprises await us," predicts Moon. "It's a pleasure to learn that we still have much that we barely understand."
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