- Open Access
A balancing act between the X chromosome and the autosomes
Journal of Biology volume 5, Article number: 2 (2006)
Dosage compensation equalizes gene dosage between males and females, but its role in balancing expression between the X chromosome and the autosomes may be far more important. Now, DNA microarrays have shown equality between the average expression of X-linked genes and that of autosomal genes, in male and female tissues of flies, worms and mice.
Sex chromosomes have evolved from an ordinary pair of autosomes many times, including in the lineages of flies, worms, mammals, and many others. In each case, the lack of recombination between the X and Y chromosomes led to loss and differentiation of genes on the Y chromosome, leaving males with a single copy of most X-linked genes . To protect organisms against deleterious effects of this X-chromosome monosomy, mechanisms of dosage compensation evolved.
The regulated dosage of any one gene is not necessarily important for the viability of an organism, but the gene dosage of a whole chromosome, or even a part of a chromosome, is vital. In Drosophila, having only one copy of (being haploid for) as little as 1% of the genome reduces viability, and being haploid for more than 3% of the genome is lethal . Given that the Drosophila X chromosome makes up about 20% of the genome, flies cannot tolerate X-chromosome deletions [2, 3]; and yet Drosophila females have two X chromosomes whereas males only have one. How is this tolerated?
An early clue to the mechanism of dosage compensation between the sexes was found in autoradiographs of salivary gland polytene chromosomes, which showed that the single X chromosome in male flies (whose genotype can be written X;AA, where A represents an autosome) is expressed at twice the level found in females (XX;AA) . A multi-protein complex termed the male-specific-lethal (MSL) complex was found to bind specifically to the male X chromosome, hyperacetylating its histone H4 at lysine 16 (H4 K16) and increasing transcription from the chromosome. In male germ cells, however, the MSL complex and H4 K16 hyperacetylation of the X chromosome are not found , and the MSL gene products are not required for the viability of the Drosophila germline [6, 7]. These findings suggest that either germ cells do not need to undergo dosage compensation, or germline dosage compensation is independent of the MSL complex. The findings of Gupta et al. now published in Journal of Biology indicate that the Drosophila germline does in fact compensate for the dosage of the X chromosome.
Gupta et al.  used microarray analysis to determine the expression of the X chromosomes and autosomes in male and female Drosophila soma and gonads. For the experiments with the soma, the authors genetically manipulated the sex-determination pathway to produce sex-transformed tissues with no germline. This elegant approach allowed them to determine the X-chromosome expression dosage without the complications caused by the sexually dimorphic expression of some genes. Furthermore, they performed a series of control experiments using mutant flies to show that changing the gene dose results in a change in expression that is easily detected by microarray analyses. They determined this using stocks with either a duplication (Dp) or a deletion (Df) of chromosome arm 2L. The resulting detected gene dose changed from 1.0 to 1.5 (in the region that has three copies in Dp/+ flies and two in Df/+ flies) and from 1.0 to 3.0 (in the region that has three copies in Dp/+ flies and one in Df/+ flies).
Having validated their approach, Gupta et al.  compared expression of the X chromosome with that of the autosomes in males and females. They found that the single X chromosome of male soma and gonads was expressed at the same level as the combined two X chromosomes of female soma and gonads; that is, the expression ratios between X chromosomes and autosomes of XX;AA female soma and X;AA male soma centered on 1. These findings confirm that, in Drosophila somatic tissues, there is a doubling of transcription from the single male X chromosome. In the germline, however, the findings of Gupta et al.  suggest that the X chromosomes in both sexes are hypertranscribed relative to autosomes, but also that the two X chromosomes of females are repressed, as the expression ratios of not only testes (X;AA) but also XX;AA ovaries and X;AA sex-transformed ovaries all centered on 1 (Figure 1).
Microarray experiments performed by Gupta et al.  on somatic tissues from Caenorhabditis elegans and mouse indicate that, in those species too, the X chromosomes are hypertranscribed relative to the autosomes. In a similar set of experiments, our lab  used microarray analyses of multiple tissue types in several mammalian species to demonstrate that the active X chromosome in both sexes is hypertranscribed. It had been predicted that once a Y-linked gene is lost during evolution, its X-linked partner would have to be hypertranscribed [1, 10]. Both microarray studies [8, 9] provide concrete evidence that it is indeed important for X-chromosome gene-expression dosage to be balanced with that of autosomes in all species (Figure 1). Although increased expression of the X chromosome seems the most logical and simple mechanism to equalize gene dosage between the X chromosome and the autosomes, decreased expression from all the autosomes would also result in balanced expression .
Perhaps the most important finding of Gupta et al.  is the fact that the X chromosomes in the Drosophila germline are upregulated to match the expression of the autosomes. The molecular mechanism of dosage compensation in these cells is a mystery, as there is no MSL complex in the germline. In the mammalian germline, our lab  found that the X chromosome was expressed but not always upregulated. But because secondary oocytes and spermatids are haploid (X;A) and primary oocytes have two active X chromosomes (XX;AA), the X chromosome would not need to be upregulated in these cells (Figure 1). Whether primary oocytes use a combination of upregulation and repression of both X chromosomes, as Gupta et al.  suggest for the Drosophila female germline, remains to be determined. In the mammalian male germline, upregulation of the X chromosome achieves dosage compensation in spermatogonia (XY;AA), and spermatocytes appear to be the only cell type with very low expression of the X chromosome compared with autosomes, owing to a silencing mechanism that inactivates unpaired chromosomes .
The finding that X-chromosome expression is upregulated in Drosophila, C. elegans, and mammals suggests that dosage compensation is essential across species. The Drosophila soma achieves this dosage compensation by selectively boosting expression from the male X chromosome. In contrast, mammals and C. elegans increase expression of the X chromosome in both males and females (or males and hermaphrodites in C. elegans). Then, to avoid having the X chromosome expressed at four times normal levels ('functional tetrasomy'), mammalian females silence one X chromosome by X inactivation, whereas C. elegans hermaphrodites decrease expression of both X chromosomes (Figure 1). Thus, mechanisms of dosage compensation differ greatly between species, probably because they evolved separately in each species out of necessity. The selective hypertranscription of the male X chromosome in the Drosophila soma may be related to the use of common pathways between sex determination and dosage compensation in this species.
Interestingly, the expression of the X chromosome seems to be slightly higher than that of autosomes in most Drosophila samples tested . Perhaps the hypertranscription of the X chromosome overcompensates and another mechanism is also needed to block or repress expression in order to achieve a perfect balance between the X chromosome and the autosomes. Higher expression of the X chromosome may also have a selective advantage related to sexual reproduction, which may account for the observed increase in X-chromosome expression in mammalian brain tissues .
Despite obvious differences, the molecular mechanisms of upregulation of the X chromosome may ultimately have features in common between species. Upregulation may have evolved gene-by-gene, through DNA sequence modifications following loss of the Y-linked gene. Alternatively, mammals and C. elegans may use a multi-protein complex for dosage compensation, as seen in Drosophila. A combination of such processes may also be at work to modulate the expression of the X chromosome in the soma and germ cells. Further studies to elucidate how each species achieves dosage compensation between the X chromosomes and the autosomes in the soma and germline will provide insights into both the evolution of sex chromosomes and the mechanisms of chromosome-wide gene regulation.
Charlesworth B: The evolution of chromosomal sex determination and dosage compensation. Curr Biol. 1996, 6: 149-162. 10.1016/S0960-9822(02)00448-7.
Lindsley DL, Sandler L, Baker BS, Carpenter AT, Denell RE, Hall JC, Jacobs PA, Miklos GL, Davis BK, Gethmann RC, et al: Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics. 1972, 71: 157-184.
Celniker SE, Wheeler DA, Kronmiller B, Carlson JW, Halpern A, Patel S, Adams M, Champe M, Dugan SP, Frise E, et al: Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster euchromatic genome sequence. Genome Biol. 2002, 3: research0079.1-0079.14. 10.1186/gb-2002-3-12-research0079.
Lakhotia SC, Mukherjee AS: Chromosomal basis of dosage compensation in Drosophila. 3. Early completion of replication by the polytene X-chromosome in male: further evidence and its implications. J Cell Biol. 1970, 47: 18-33. 10.1083/jcb.47.1.18.
Rastelli L, Kuroda MI: An analysis of maleless and histone H4 acetylation in Drosophila melanogaster spermatogenesis. Mech Dev. 1998, 71: 107-117. 10.1016/S0925-4773(98)00009-4.
Schüpbach T: Normal female germ cell differentiation requires the female X chromosome to autosome ratio and expression of Sex-lethal in Drosophila melanogaster. Genetics. 1985, 109: 529-548.
Bachiller D, Sanchez L: Mutations affecting dosage compensation in Drosophila melanogaster: effects in the germline. Dev Biol. 1986, 118: 379-384. 10.1016/0012-1606(86)90007-2.
Gupta V, Parisi M, Sturgill D, Nuttall R, Doctolero M, Dudko OK, Malley JD, Eastman PS, Oliver B: Global analysis of X-chromosome dosage compensation. J Biol. 2006, 5: 3-
Nguyen DK, Disteche CM: Dosage compensation of the active X chromosome in mammals. Nat Genet. 2006, 38: 47-53.
Ohno S: Sex Chromosomes and Sex-Linked Genes. 1967, Berlin: Springer-Verlag
Bhadra MP, Bhadra U, Kundu J, Birchler JA: Gene expression analysis of the function of the male-specific lethal complex in Drosophila. Genetics. 2005, 169: 2061-2074. 10.1534/genetics.104.036020.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Cheng, M.K., Disteche, C.M. A balancing act between the X chromosome and the autosomes. J Biol 5, 2 (2006). https://doi.org/10.1186/jbiol32
- Dosage Compensation
- Primary Oocyte
- Salivary Gland Polytene Chromosome
- Drosophila Germline
- Time Normal Level